Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1997-04-08
1998-12-01
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 32, 435970, 435973, 4353081, 435 23, 435 24, 435 16, 536 13, 536 111, C12Q 104, C12Q 100, C12Q 118, G01N 3353
Patent
active
058436995
ABSTRACT:
A rapid screening method for the detection and identification of target microorganisms in a sample which may contain both target microorganisms and competing non-target microorganisms includes the steps of enriching a sample in a mildly selective medium, incubating the sample in the growth medium for a predetermined amount of time, adding at least one inhibitor of the non-target microorganisms to the growth medium to discourage the growth of non-target microorganisms and to encourage the growth of target microorganisms. The method further includes the steps of incubating the sample in the growth medium including at least one inhibitor for a predetermined amount of time, performing biochemical assays specific for the identification of the target microorganisms, and detecting the presence of the target microorganisms in the sample. The invention further provides a system for the rapid detection of microorganisms in a sample.
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Nagar Mandar S.
Schneider Maureen A.
Strenkoski Leon F.
Swamy Shoba C.
Difco Laboratories, Inc.
Leary Louise N.
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