Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1996-01-04
1998-04-21
Leary, Louise
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 24, 435 34, 435 16, 435 13, 435 25, 435 4, 514 2, 514 70, 514740, 564305, C12Q 137, C12Q 104, C12Q 152, A01N 3718
Patent
active
057416591
ABSTRACT:
An assay for detecting microbial protease activity in clinical and laboray samples is described which comprises gathering a sample suspected of containing certain microorganisms having the desired protease activity; immobilizing the microorganisms in the sample on a solid phase substrate; contacting the immobilized microorganisms with an enzymatic substrate producing an enzymatic substrate end-product; contacting the enzymatic substrate end-product with a chemical enhancing reagent producing a detectable chromogenic reaction which varies in intensity with the level of protease activity in the sample; and detecting the chromogenic reaction whereby the semi-quantitative presence of the protease activity in the sample is determined. The device for conducting these assays is a frame or support which holds a solid phase substrate capable of binding the sought microorganisms of interest while permitting drainage of other materials or fluids, which may contain host proteases, away from the immobilized microorganisms.
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Ralls Stephen Alden
Schade Sylvia Zottu
Simonson Lloyd Grant
Garuert W.
Leary Louise
Spevack A. D.
The United States of America as represented by the Secretary of
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