Rapid method to detect duplex formation in sequencing by hybridi

Details Rapid method to detect duplex formation in sequencing by hybridi Rapid method to detect duplex formation in sequencing by hybridi

1996-01-26

1999-01-19

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, C12Q 168

Patent

active

058612475

ABSTRACT:
A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to coalesce into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units.

REFERENCES:
patent: 5552270 (1996-09-01), Khrapko et al.
patent: 5582984 (1996-12-01), Bieniarz et al.
Southern, et al., Analyzing and Comparing Nucleic Acid Sequences by Hybridization to Arrays of Oligonucleotides: Evaluation Using Experimental Models, Genomics 13:1008-1017, 1992.

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