Rapid method for the detection of beta-lactamase in body fluids

Details Rapid method for the detection of beta-lactamase in body fluids Rapid method for the detection of beta-lactamase in body fluids

1985-06-17

1988-07-26

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 18, G01N 33566, C12Q 134

Patent

active

047600187

ABSTRACT:
A rapid method for the detection of beta-lactamase in body fluids which method comprises contacting a sample of a body fluid with charcoal, removing the charcoal and recovering an essentially charcoal free supernatant, adding a labeled beta-lactam compound such as C.sup.14 penicillin to the supernatant for a defined period of time, adding a antibiotic sensitive microorganism, incubating and thereafter removing the microorganism cells from the incubation broth, measuring the amount of C.sup.14 bound-labeled penicillin and comparing the measured amount with a controlled sample or standard data or graph to determine the amount of any beta-lactamase in the body fluid sample.

REFERENCES:
patent: 4238521 (1980-12-01), Charm
patent: 4239745 (1980-12-01), Charm
patent: 4239852 (1980-12-01), Charm
patent: 4353824 (1982-10-01), Schindler
patent: 4390622 (1983-06-01), Cartwright
patent: 4448880 (1984-05-01), Schindler et al.
Yolken et al., J. Pediatrics, 97(5): 715-719 (1980).
Scudamore et al., Can. J. Microb., 22(1): 76-82 (1976).

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