Rapid method for preparing chromogenic alpha-amylase substrate c

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 22, 435 96, 435810, C12P 1918

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active

049328719

ABSTRACT:
A method for preparing chromogenic .alpha.-amylase substrate compounds at substantially high preparative yields in a relatively short period of time by the transglucosylation of a chromogenic glucoside compound to a maltooligosaccharide chain. The concentration of the maltooligosaccharide compound is greater than about 3 times the concentration of the chromogenic glucoside compound to result in at least about 50% of the chromogenic glucoside compound being converted to the desired chromogenic .alpha.-amylase substrate compound.

REFERENCES:
patent: 3694318 (1972-09-01), Klein
patent: 4169765 (1979-10-01), Keyes
patent: 4225672 (1980-09-01), Hall
patent: 4237221 (1980-12-01), Helmgard
patent: 4505756 (1985-03-01), Nix

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