Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Amino acid or sequencing procedure
Patent
1985-07-31
1987-05-19
Marcus, Michael S.
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
Amino acid or sequencing procedure
2041826, 435 4, 436163, 436164, C12Q 100, G01N 3368
Patent
active
046668551
ABSTRACT:
A rapid method for determining the isoelectric point for an amphoteric molecule of interest is provided which utilizes the pH-dependent binding affinity of the molecule for an ion-exchange material. The empirically derived pI values are within a range of 0.2 pH units or less of the reported values in the literature and may be more precisely determined by employing a narrower pH gradient as part of the procedure. The unique methodology allows isoelectric point determinations to be accomplished within one hour's time, avoids carrier ampholyte interaction or artifact formation, and allows the determination to be performed at any desired temperature with decreased risk of denaturation.
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Langer Robert S.
Yang Victor C.
Cook Paul J.
Marcus Michael S.
Massachusetts Institute of Technology
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