Rapid membrane affinity concentration assays

Details Rapid membrane affinity concentration assays Rapid membrane affinity concentration assays

1992-07-31

1993-04-27

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 71, 435 75, 435 792, 435 793, 435 794, 435 795, 435180, 435803, 435971, 435974, 436501, 436539, 436810, G01N 33538, C12Q 100

Patent

active

052061361

ABSTRACT:
Rapid assays for analytes of interest in a fluid sample utilize a first conjugate of a labelled reactant that specifically binds to the analyte, and a second conjugate that binds to the analyte coupled to a polymer that has an affinity for a selected solid phase. The reaction components are incubated briefly, then contacted with the selected solid phase and the labelled components determined. Optional wash steps provide for enhanced sensitivity and specificity. When the analyte of interest is an antibody to HIV, the first reactant may be a synthetic, recombinant or native HIV antigen, and the second reactant may be protein A or an anti-immunoglobulin.

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