Rapid means of quantitating genomic instability

Details Rapid means of quantitating genomic instability Rapid means of quantitating genomic instability

1996-10-22

1999-06-15

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 6, 536 2433, 935 8, 935 77, 935 78, C12P 1934, C12Q 168, C07H 2104

Patent

active

059121475

ABSTRACT:
Provided is a method for detecting genomic instability, independent of microsatellite alterations, comprising the steps of treating a comparison pair of genomic DNA from tumor cells and genomic DNA from normal cells with oligonucleotide primers under conditions for, and in an amplification reaction to, amplify a target sequence associated with such genomic instability. Detection of an alteration in the banding pattern of amplified product from genomic DNA from tumor cells as compared to that of normal cells, is indicative of such genomic instability. Additionally provided is a process for simultaneously assessing changes in methylation patterns in the genomic DNA tested comprising treating the genomic DNA with reagents for detecting methylation status prior to the amplification reaction, comparing the resultant banding patterns of amplified products, wherein differences in the banding patterns is indicative of an alteration in methylation. Disclosed are novel compositions for use in a method for detecting genomic instability, independent of microsatellite alterations.

REFERENCES:
Zietkiewicz et al., "Genome Fingerprinting by Simple Sequence Repeat (SSR)-Anchored Polymerase Chain Reaction Amplification", 1994, Genomics, vol. 20, pp. 176-183.
Cross et al. "CpG islands & genes" Current Biology 5:309-314 (Jun. 1995).
Makos et al. Proc.Natl.Acad. USA. 89:1929-1933 (Mar. 1992).
Sheardy. Nucl.Acids. Res. 16:1153-1167 (Feb. 1988).

Affiliated with

Also associated with

Rating

Rapid means of quantitating genomic instability does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Rapid means of quantitating genomic instability, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid means of quantitating genomic instability will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-401997