Rapid isolation of osteoinductive protein mixtures from...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins from mammals or birds – Bones; tendons; teeth; cartilage

Reexamination Certificate

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C530S412000, C514S021800, C424S549000

Reexamination Certificate

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10606190

ABSTRACT:
A method for purifying bone-derived osteogenic proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recovery process. The high and low ultrafiltration processes preferably select proteins having a nominal molecular weight between approximately 8 kilodaltons and approximately 50 kilodaltons. Processes of the present invention may be used to recover osteogenic proteins from bone demineralization waste streams.

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