Rapid, immunochemical process for measuring thiopurine...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S007100, C435S007920, C435S007100, C436S517000

Reexamination Certificate

active

06946258

ABSTRACT:
The present invention relates to non-isotopic immunoassays for thiopurine methyltransferase (TPMT). The immunoassays of this invention may be homogenous or heterogenous, in which detection of the TPMT-catalyzed reaction product relies upon specific binding of antibody to 6-MMP or other TPMT-catalyzed reaction products. Preferred embodiments of this invention include a Rapid Immunomigration Cassette and an assay carried out in ELISA assay format.

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Kroplin et al, Thiopurine S-Methyltransferase activity in human erythrocytes: a new HPLC method using 6-thioguanine as substrate, European Journal of Clinical Pharmacology (1998), vol. 54, pp. 265-271.
Micheli et al, Thiopurine methyltransferase activity in the erythrocytes of adults and children: an HPLC-linked assay, Clinica chimica Acta, (1997) vol. 259, pp. 161-168.
Pressman et al.; “The Serological Properties of Simple Substances. IV. Hapten Inhibition of Precipitation of Antibodies and Polyhaptenic Simple Substances”; Hapten Inhibition of Precipitation of Antibodies and Polyhaptens; Jam. Chem. Soc. vol. 64—pp. 3015-3020 (1942).
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