Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1996-12-12
2000-01-18
Caputa, Anthony C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 734, 435 79, 435 792, 435 35, 435975, 436518, 436524, 436530, 436548, G01N 33554, G01N 3353, G01N 33537, G01N 33543
Patent
active
060156813
ABSTRACT:
An assay method and kit for detecting specific oral cariogenic bacteria, ., mutans streptococci, Lactobacillus sp. and Actinomyces sp., separately or in combination, comprising gathering a sample suspected of containing cariogenic bacteria; treating the sample with a stripping buffer to remove host antibodies from bacteria present in the sample; retaining the treated bacteria on a blocked solid phase substrate; reacting the retained bacteria with a primary antibody specific for the desired cariogenic bacteria; reacting the primary antibody with a conjugated label producing a detectable signal; and detecting the signal whereby the presence of the desired cariogenic bacteria is determined in the sample. The device for conducting these assays is a frame or support which holds a solid substrate capable of retaining the bacteria of interest while permitting drainage of other materials or fluids away from the retained bacteria.
REFERENCES:
patent: 4632901 (1986-12-01), Valkirs et al.
patent: 5017342 (1991-05-01), Haberzettl et al.
patent: 5103836 (1992-04-01), Goldstein et al.
Kinet et al(Biochemistry vol. 24, pp. 4117-4124), 1985.
Ralls Stephen Alden
Simonson Lloyd Grant
Caputa Anthony C.
Navarro Mark
Spevack A. D.
The United States of America as represented by the Secretary of
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