Rapid identification of microbial organisms and determination of

Details Rapid identification of microbial organisms and determination of Rapid identification of microbial organisms and determination of

1990-06-18

1992-05-12

Nucker, Christine

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435 32, 435 34, C12Q 102, C12Q 118, C12Q 104

Patent

active

051127458

ABSTRACT:
A rapid and effective method is provided for determining various genotypic and/or phenotypic characteristics of an unknown microbial organism in a non-destructive manner. Initially, a predetermined growth medium is inoculated with an unknown microbial organism to create a microbial culture and the culture is then scanned at a time after inoculation to obtain a spectral signature. This scanning step is conducted utilizing wavelengths over a portion of the electromagnetic spectrum from 700-5000 nm. Thereafter, characterizing data of the unknown organism is determined from the spectral signature. This characterizing data of the unknown organism is compared with a preexisting library of characterizing data of known organisms with known characteristics, which library was made by scanning known organisms in the same manner as the unknown organism, to determine if there is a match between the characterizing data of the unknown organism and the characterizing data of a known organism contained in the library. When the characteristic of the unknown organism is matched, the identity of the unknown organism has been found. The present method is advantageous in that identity, quantity, growth rate and antibiotic sensitivity of an unknown microorganism can be easily and reliably determined more quickly than would be possible using previously known methods.

REFERENCES:
patent: 4017192 (1977-04-01), Rosenthal
patent: 4384206 (1983-05-01), Bjarno
patent: 4725148 (1988-02-01), Endo et al.
patent: 4758509 (1988-07-01), Ottley
patent: 4847198 (1989-07-01), Nelson et al.
Solomons (1980) Organic Chemistry Wiley Co N.Y. pp. 558-574.

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