Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1994-04-21
1998-03-10
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 935 77, C12Q 170, C12Q 168
Patent
active
057260125
ABSTRACT:
A rapid, high capacity method using chaotropic agents for evaluating nucleic acids is described. Samples containing the target nucleic acid can be evaluated in a nucleic acid based sandwich hybridization assay which is performed, in part, in a chaotropic solution which is removed prior to detecting and/or quantitating the product. This assay can be used to detect and/or quantitate nucleic acid levels. It can also be used as an infectivity assay and/or as an assay to evaluate anti-infectious agent activity of compounds.
REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4563419 (1986-01-01), Ranki et al.
patent: 4581333 (1986-04-01), Kourilsky et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4711955 (1987-12-01), Ward et al.
patent: 4994373 (1991-02-01), Stavrianopoulos et al.
W.R. Hunsaker, H. Badri, M. Lombardo, & M.L. Collins, Nucleic Acid Hybridization Assays Employing dA-Tailed Capture Probes, Analytical Biochemistry, 181, 360-370, 1989.
D.V. Morrissey, M. Lombardo, J.K. Eldredge, K.R. Kearney, C.P. Groody, & M.L. Collins, Nucleic Acid Hybridization Assays Employing dA-Tailed Capture Probes, Analytical Biochemistry, 181, 345-359, 1989.
Matthews et al. Analytical Biochemistry 169: 1-5 (1988).
Pellegrino et al, A Sensitive Solution Hybridization Technique for Detecting RNA in Cells: Application to HIV in Blood Cells, BioTechniques, vol. 5, No. 5, 452-458, 1987.
Ness, et al, The Use of Oligodeoxynucleotide Probes in Chaotrope-based Hybridization Solutions, Nucleic Acids Research, vol. 19, No. 19, 5143-5151, Sep. 4, 1991.
Richardson, et al, Biotin and Fluorescent Labeling of RNA Using T4 RNA Ligase, Nucleic Acids Research, vol. 11, No. 18, 6167-6184, Aug. 30, 1983.
Thompson, et al., Enzymatic Amplification of RNA Purified from Cured Cell Lysate by Reversible Target Capture, Clin. Chem. vol. 35, No. 9, 1878-1881, 1989.
Molecular Cloning: A Laboratory Manual, Maniatis et al., Cold Spring Harbor Laboratory (1982), pp. 390-401.
Dunn et al., `A Novel Method to Map Transcripts: Evidence for Homology between an Adenovirus mRNA and Discrete Multiple Regions of the Viral Genome`, Cell, vol. 12, pp. 23-26 (Sep. 1977).
Thompson et al., `Enzymatic Amplification of RNA Purified from Crude Cell Lysate by Reversible Target Capture`, Clinical Chemistry, vol. 35, No. 9, pp.1878-1881 (1989).
Pellegrino et al., `A Sensitive Solution Hybridization Technique for Detecting RNA in Cells: Application to HIV in Blood Cells`, Research Report, vol. 5, No. 5 (1987).
Gillespie et al., `Probes for quantitating subpicogram amounts of HIV-1 RNA by molecular hybridization`, Molecular and Cellular Probes (1989) vol. 3, pp. 73-86.
Thompson et al., `Molecular Hybridization with RNA Probes in Concentrated Solutions of Guanidine Thiocyanate`, Analytical Biochemistry, vol. 163, pp. 281-291 (1987).
Albretsen et al., `Applications of Magnetic Beads with Covalently Attached Oligonucleotides in Hybridization: Isolation and Detection of Specific Measles Virus mRNA from a Crude Cell Lysate`, Analytical Biochemistry, vol. 189, pp. 40-50 (1990).
Volsky et al., `Titration of human immunodeficiency virus type 1 (HIV-1) and quantitative analysis of virus expression in vitro using liquid RNA-RNA hybridization`, Journal of Virological Methods, vol. 28, pp. 257-272 (1990).
Solomon et al., `Quantitation of HIV-1 RNA in Blood Cels of ARC and AIDS Patients`, Journal of Clinical Laboratory Analysis, vol. 3, pp. 282-286 (1989).
Japour et al., `Detection of human immunodeficiency virus type 1 clinical isolates with reduced sensitivity to zidovudine and dideoxyinosine by RNA-RNA hybridization`, Proc. Natl. Acad. Sci. USA, vol. 88, pp. 3092-3096 (Apr. 1991) Medical Sciences.
Decker et al., `Quantitative Analysis of HIV-1 Expression Using Magnetic Particle RNA-RNA Hybridization: Application to Anti-HIV Drug Screening`, International Conference on AIDS: The Scientific and Social Challenge, Quebec, Canada (Jun. 4-9, 1989).
Bacheler Lee Terry
Miller Jeffrey Allan
Stone Barry Allen
E. I. Du Pont de Nemours and Company
Zitomer Stephanie W.
LandOfFree
Rapid, high capacity nucleic acid based assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rapid, high capacity nucleic acid based assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid, high capacity nucleic acid based assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-138181