Rapid flow fractionation of particles combining liquid and...

Chemistry: electrical and wave energy – Processes and products – Electrophoresis or electro-osmosis processes and electrolyte...

Reexamination Certificate

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C204S643000

Reexamination Certificate

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11189123

ABSTRACT:
Rapid, size-based, deposition of particles from liquid suspension is accomplished using a nonuniform electric field created by coplanar microelectrode strips patterned on an insulating substrate. The scheme uses the dielectrophoretic force both to distribute aqueous liquid containing particles and, simultaneously, to separate the particles. Size-based separation is found within nanoliter droplets formed along the structure after voltage removal. Bioparticles or macromolecules of similar size can also be separated based on subtle differences in dielectric property, by controlling the frequency of the AC current supplied to the electrodes.

REFERENCES:
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patent: 6641708 (2003-11-01), Becker et al.
patent: 6766817 (2004-07-01), da Silva
patent: 7029564 (2006-04-01), Lock et al.
patent: 7105081 (2006-09-01), Gascoyne et al.
Jones et al. (“Dielectrophoretic liquid actuation and nanodroplet formation,” Journal of Applied Physics, vol. 89, No. 2, 15 Jan. 2001).
Pohl et al. (“The Continuous Positive and Negative Dielectrophoresis of Microorganisms,” J. Biol. Phys. vol. 9, 1981, p. 67-86).
M. R. King, et al., “Size-Selective Deposition of Particles Combining Liquid and Particulate Dielectrophoresis”, Journal of Applied Physics, 97, 054902 (2005).

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