Rapid extraction of RNA from cells and tissues

Details Rapid extraction of RNA from cells and tissues Rapid extraction of RNA from cells and tissues

2007-09-11

2007-09-11

Kim, Young J. (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

Reexamination Certificate

active

10427217

ABSTRACT:
A method of extracting RNA from biological systems (cells, cell fragments, organelles, tissues, organs, or organisms) is presented in which a solution containing RNA is contacted with a substrate to which RNA can bind. RNA is withdrawn from the substrate by applying negative pressure. No centrifugation step exceeds thirty seconds. Preferably, the RNA is diluted prior to filtration and one or more wash steps can be used to remove interferents. In one such method, except for DNA shearing and drying steps, centrifugation is not undertaken during the extraction step.

REFERENCES:
patent: 3389133 (1968-06-01), Gutcho
patent: 4843155 (1989-06-01), Chomczynski
patent: 5010183 (1991-04-01), Macfarlane
patent: 5155018 (1992-10-01), Gillespie
patent: 5346994 (1994-09-01), Chomczynski
patent: 5438128 (1995-08-01), Nieuwkerk et al.
patent: 5620852 (1997-04-01), Lin et al.
patent: 5643767 (1997-07-01), Fischetti et al.
patent: 5654179 (1997-08-01), Lin
patent: 5777099 (1998-07-01), Mehra
patent: 5786208 (1998-07-01), Clark et al.
patent: 5945515 (1999-08-01), Chomczynski
patent: 5973137 (1999-10-01), Heath
patent: 6204375 (2001-03-01), Lader
patent: 6218531 (2001-04-01), Ekenberg
patent: 6248535 (2001-06-01), Danenberg et al.
patent: 6428963 (2002-08-01), Danenberg et al.
patent: 2002/0009738 (2002-01-01), Houghton et al.
patent: 2002/0009794 (2002-01-01), Danenberg et al.
patent: 2002/0009795 (2002-01-01), Danenberg et al.
patent: 2002/0081619 (2002-06-01), Bastian et al.
patent: WO95/28409 (1995-10-01), None
patent: WO98/45311 (1998-10-01), None
patent: WO 02/057289 (2002-07-01), None
patent: WO 02/070751 (2002-09-01), None
Johansson et al., “Quantitative Analysis of Tyrosinase Transcripts in Blood,” Clinical Chemistry, 2000, vol. 46, No. 7, pp. 921-927.
Ultraspec-3 RNA, “Isolation system for liquid samples,” Product description, published and available as of Feb. 1999.
European Search Report, dated Jul. 20, 2005, for European Appln. No. EP04252529.
Ambion, Inc., “RNAqueous TM-4PCR Instruction Manual, Version 0204”, RNAQUEOUSTM-4PCR, Apr. 2002, pp. 1-29.
Quiagen, Inc., “RNeasy Mini Handbook”, RNeasy, Mini, Jun. 2001, pp. 1-112.
“Maximize your RNA yield: What yield to expect”, Technotes International Edition, vol. 8, No. 3, May 2001, pp. 1, 13-14, Austin Texas.

Affiliated with

Also associated with

Rating

Rapid extraction of RNA from cells and tissues does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Rapid extraction of RNA from cells and tissues, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid extraction of RNA from cells and tissues will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3725233