Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined...
Patent
1992-05-01
1994-02-15
Rollins, John W.
Drug, bio-affecting and body treating compositions
Extract, body fluid, or cellular material of undetermined...
514561, 536 185, 435 721, A61K 3512, A61K 31195, C07H 1500
Patent
active
052864987
ABSTRACT:
The present invention relates to methods for the rapid extraction of ciguatoxins from tissue samples. In one embodiment, a tissue sample is extracted with a first solvent to solubilize and extract ciguatoxin from the sample. A second solvent, which is not soluble in the first solvent and which extracts contaminants but not ciguatoxin, is then added. The phases formed by the first and second solvents are separated, and the first solvent phase is collected. The first solvent is evaporated from the first solvent phase to produce a residue which is resuspended in a third solvent. A fourth solvent, which is not soluble in the third solvent and which extracts contaminants but not ciguatoxin, is added to the third solvent/residue mixture. The phases formed by the third and fourth solvents are separated, and the third solvent phase, which comprises ciguatoxin, is collected. In a second embodiment, a sample is applied to a coupled antibody-resin. The coupled antibody-resin is washed with a solution to remove unbound material, but not ciguatoxin, from the coupled antibody-resin, and ciguatoxin is then eluted from the coupled antibody-resin.
REFERENCES:
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Gamboa Pedro M.
Park Douglas L.
Hawaii Chemtect Incorporated
Rollins John W.
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