Rapid, easy, and economical screening test for antibodies to hum

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage

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435 792, 435974, 435975, 435 72, C12Q 170, C12Q 100, G01N 3353

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active

051496232

ABSTRACT:
A new dot enzyme immunoassay (EIA) with a conserved portion of the envelope protein of the human immunodeficiency virus (HIV) as antigen has been designed for use in areas with few laboratory facilities and by personnel with little laboratory experience. Sera were tested in 263 subjects who had AIDS or AIDS-related complex or were at -risk or not-at-risk of AIDS from the USA, Africa, and Asia/Oceania. The dot EIA was 100% sensitive in the American subjects, and there were only 2 false negatives in the others, both of which were negative by commerical EIA. The test is simple to perform, economical, rapid (30 min), and stable.

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