Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1994-10-28
1997-09-16
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 4, C12Q 170, C12Q 100
Patent
active
056679645
ABSTRACT:
The ability to monitor the progression of human immunodeficiency virus (HIV) infection in patients is paramount to the study of HIV transmission, in predicting the onset and advancement of disease, and evaluating the clinical efficacy of therapeutics. Present methods available to the clinician for the study of HIV pathogenesis employ surrogate markers. Surrogate markers are biological indicators that tend to reflect, to varying extent, the gradual progression of the asymptomatic state to the development of acquired immune deficiency syndrome (AIDS). The most commonly used markers are CD4.sup.+ lymphocyte counts and HIV p24 antigen production. The use of markers to evaluate disease progression suffers from a number of limitations. No known marker consistently reflects disease progression in all patients and stages of disease. Moreover, an effective marker must rapidly reflect the changes associated with antiviral therapy. Accordingly, there still exists in the field a need for a rapid and direct technique for assessing the viral load of an HIV-infected patient. The present invention discloses a rapid method for qualitatively determining the number of HIV-1-infected patient cells in a sample. Patient cells are subjected to direct stimulation with reactive oxygen intermediate generator(s), in the absence of co-culture with donor cells, and the quantity of p24 antigen produced from said stimulation ascertained. These values are compared to those values obtained from HIV-1 chronically infected cell lines of known proviral copy number (e.g., ACH-2 and U1.1) and the number of infected patient cells determined. This method provides a facile, rapid, and direct method for the assessment of viral load.
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Cornell Research Foundation Inc.
Nucker Christine M.
Parkin Jeffrey S.
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