Rapid diagnostic methods of Peste des Petits Ruminants using...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Animal cell – per se – expressing immunoglobulin – antibody – or...

Reexamination Certificate

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C435S005000, C435S007920

Reexamination Certificate

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11261611

ABSTRACT:
The present invention relates to a diagnostic method of Peste des Petits Ruminants (PPR), a viral animal disease. More specifically, the invention relates to a new diagnostic method capable of detecting PPRV antibodies safely, simply and rapidly with recombinant Nucleocapsid (N) protein of PPR virus (PPRV) and specific monoclonal antibody against N protein from PPRV.

REFERENCES:
Choi et al, Monoclonal antibody-based competitive ELISA for simultaneous detection of rinderpest virus and peste des petits ruminants virus antibodies, Veterinary Microbiology, 2003, vol. 96, p. 1-16. (See IDS).
Libeau et al., A competitive ELISA using anti-N monoclonal antibodies for specific detection of rinderpest antibodies in cattle and small ruminants, Veterinary Microbiology, 1992, vol. 31, p. 147-160.
Sinnathamby et al., Immune responses in goats to recombinant hemagglutinin-neuraminidase glycoproteins of Peste des Petits ruminats virus: identification of a T cell determinant, Vaccine, 2001, vol. 19, p. 4816-4823.
Singh et al., Development of a monoclonal antibody based aompetitive-ELISA for detection and titration of antibodies to peste des petits ruminants (PPR) virus, Veterinary Microbiology, 2004, vol. 98, p. 3-15.
G. Libeau, “Development of a competitive ELISA for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleoprotein”, Research in Veterinary Science, (1995), 58, pp. 50-55.
K. Choi et al., “Monoclonal antibody-based competitive ELISA for simulatneous detection of rinderpest virus and pese des petits ruminants virus antibodies”, Veterinary Microbiology 96, (2003), pp. 1-16.
T. Ismail et al., “Cloning and Expression of the Nucleoprotein of Peste des Petits Ruminants Virus in Baculovirus for Use in Serological Diagnosis”, Virology 208, (1995), pp. 776-778.

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