Rapid detection of virulence-associated factors

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 735, 435 737, 435 74, 435 792, 435962, 436530, 436177, 436825, G01N 33569, G01N 33543, G01N 3362, G01N 33573

Patent

active

055522947

ABSTRACT:
A method for detecting at least one virulence-associated factor (VAF), e.g., a bacterial toxin, in a sample is described. The sample suspected of containing the VAF-producing bacteria is contacted with a VAF releasing solution under conditions which release VAF from the bacteria. The released VAF subsequently is immunochemically detected. The preferred method is a membrane-based enzyme linked immunosorbent assay for immunochemically detecting the well-characterized Shiga family toxins including SLT I and SLT II. Also described is the VAF releasing solution and a kit containing the reagents for conducting the described methods.

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