Rapid detection of mutations in the p53 gene

Details Rapid detection of mutations in the p53 gene Rapid detection of mutations in the p53 gene

1995-06-07

1998-12-01

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435199, C12Q 168, C12P 1934

Patent

active

058436545

ABSTRACT:
The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5' nucleases and 3' exonucleases, are used to screen for known and unknown mutations, including single base changes, in the human p53 gene. Methods are provided which allow for the identification of genetic mutations in the human p53 gene in a sample.

REFERENCES:
patent: 4511502 (1985-04-01), Builder et al.
patent: 4511503 (1985-04-01), Olson et al.
patent: 4512922 (1985-04-01), Jones et al.
patent: 4518526 (1985-05-01), Olson
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5108892 (1992-04-01), Burke et al.
patent: 5144019 (1992-09-01), Rossi et al.
patent: 5210015 (1993-05-01), Gelfand et al.
patent: 5422253 (1995-06-01), Dahlberg et al.
patent: 5455170 (1995-10-01), Abramson, et al.
patent: 5541311 (1996-07-01), Dahlberg et al.
patent: 5614402 (1997-03-01), Dahlberg et al.
Barany, "Genetic disease detection and DNA amplification using cloned thermostable ligase," Proc. Natl. Acad. Sci., 88:189 (1991).
Barany, F., "The Ligase Chain Reaction in a PCR World," PCR Methods and Applic., 1:5-16 (1991).
Wu and Wallace, "The Ligation Amplification Reaction (LAR)--Amplification of Specific DNA Sequences Using Sequential Rounds of Template-Dependent Ligation," Genomics 4:560 (1989).
Guatelli et al., "Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication," Proc. Natl. Acad. Sci., 87:1874-1878 (1990).
Erratum: Guatelli et al., "Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication," Proc. Natl. Acad. Sci., 87:7797 (1990).
Kwoh et al., "Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format," Proc. Natl. Acad. Sci., 86:1173-1177 (1989).
Fahy et al., "Self-sustained Sequence Replication (3SR): An Isothermal Transcription-based Amplification System Alternative to PCR," PCR Meth. Appl., 1:25-33 (1991).
Landgren, "Molecular mechanics of nucleic acid sequence amplification," Trends in Genetics 9:199 (1993).
Mullis, "The Polymerase Chain Reaction in an Anemic Mode: How to Avoid Cold Oligodeoxyribonuclear Fusion," PCR Methods Applic., 1:1 (1991).
S. Kwok et al., "Effects of Primer-Template Mismatches on the Polymerase Chain Reaction: Human Immunodeficiency Virus Type 1 Model Studies," Nucl. Acids Res. 18:999 (1990).
Duck et al., "Probe Amplifier System Based on Chimeric Cycling Oligonucleotides," BioTech., 9:142 (1990).
Urdea et al., "A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity; application to the analysis of hepatitis B virus in human serum," Gene 61:253-264 (1987).
Gogos et al., "Detection of single base mismatches of thymine and cytosine residues by potassium permanganate and hydroxylamine in the presence of tetralkylammonium salts," Nucl. Acids Res., 18:6807-6817 (1990).
Barlow and Lehrach, "Genetics by gel electrophoresis: the impact of pulsed field gel electrophoresis on mammalian genetics," Trends Genet., 3:167 (1987).
Perlman and Butow, "Mobile Introns and Intron-Encoded Proteins," Science 246:1106 (1986).
Conner et al., "Detection of sickle cell .beta..sup.S -globin allele by hybridization with synthetic oligonucleotides," Proc. Natl. Acad. Sci., 80:278-282 (1983).
Vogelstein et al., "Genetic Alternations During Colorectal-Tumor Development," N. Eng. J. Med., 319:525-532 (1988).
Farr et al., "Analysis of RAS gene mutations in acute myeloid leukemia by polymerase chain reaction and oligonucleotide probes," Proc. Natl. Acad. Sci., 85:1629-1633 (1988).
Lyons et al., "Two G Protein Oncogenes in Human Endocrine Tumors," Science 249:655-659 (1990).
Abrams et al., "Comprehensive Detection of Single Base Changes in Human Genomic DNA Using Denaturing Gradient Gel Electrophoresis and a GC Clamp," Genomics 7:463-475 (1990).
Sheffield et al., "Attachment of a 40-base-pair G+C-rich sequence (GC-clamp) to genomic DNA fragments by the polymerase chain reaction results in improved detection of single-base changes," Proc. Natl. Acad. Sci., 86:232-236 (1989).
Lerman and Silverstein, "Computational Simulation of DNA Melting and Its Application to Denaturing Gradient Gel Electrophoresis," Meth. Enzymol., 155:482-501 (1987).
Wartell et al., "Detecting base pair substitutions in DNA fragments by temperature-gradient gel electrophoresis," Nucl. Acids Res., 18:2699-2701 (1990).
Smith et al., "Novel Method of Detecting Single Base Substitutions in RNA Molecules by Differential Melting Behavior in Solution," Genomics 3:217-223 (1988).
Borresen et al., "Constant denaturant gel electrophoresis as a rapid screening technique for p53 mutations," Proc. Natl. Acad. Sci. USA 88:8405 (1991).
Scholz, et al., "Rapid screening for Tp53 mutations by temperature gradient gel electrophoresis: a compairson with SSCP analysis," Hum. Mol. Genet. 2:2155 (1993).
K. Hayashi, "PCR-SSCP: A Simple and Sensistive Method for Detection of
M. Orita, et al., "Rapid and Simple Detection of Point Mutations and DNA Polymorphisms Using the Polymerase Chain Reaction," Genomics 5:874-879
Liu and Sommer, "Parameters Affecting the Sensitivity of Dideoxy Fingerprinting and SSCP," PCR Methods Appl. 4:97 (1994).
A. Kornberg, DNA Replication, W.H. Freeman and Co., San Francisco, pp. 127-139 (1980).
K.R. Tindall and T.A. Kunkel, "Fidelity of DNA Synthesis by the Thermus aquaticus DNA Polymerase," Biochem. 27:6008 (1988).
D. Brutlag et al., "An Active Fragment of DNA Polymerase Produced by Proteolytic Clevage," Biochem. Biophys. Res. Commun. 37:982 (1969).
H.A. Erlich et al., "Recent Advances in the Polymerase Chain Reaction," Science 252:1643 (1991).
P. Setlow and A. Kornberg, "Deoxyribonucleic Acid Polymerase: Two Distinct Enzymes in One Polypeptide--II. A Proteolytic Fragment Containing the 5'-3'Exonuclease Function. Restoration of Intact Enzyme Functions from the Two Proteolytic Fragments," J. Biol. Chem. 247:232 (1972).
D.H. Gelfand, PCR Technology--Principles and Applications for DNA Amplification (H.A. Erlich, Ed.), Stockton Press, New York, p. 19 (1989).
P.M. Holland et al., "Detection of Specific Polymerase Chain Reaction Product by Utilizing the 5'-3' Exonuclease Activity of Thermus aquaticus DNA Polymerase," Proc. Natl. Acad. Sci. USA 88:7276 (1991).
J. Marmur and D. Lane, "Strand Separation and Specific Recombination in Deoxyribonucleic Acids: Biological Studies," Proc. Natl. Acad. Sci USA 46:453 (1960).
P. Doty et al., "Strand Separation and Specific Recombination in Deoxyribonucleic Acids: Physical Chemical Studies," Proc. Natl. Acad. Sci. USA 46:461 (1960).
R. B. Wallace et al., "Application of Synthetic Oligonucleotides to the Diagnosis of Human Genetic Diseases," Biochimie 67:755 (1985).
A. B. Studencki and R. B. Wallace, "Allele-Specific Hybridization Using Oligonucleotide Probes of Very High Specific Activity: Discrimination of the Human .beta..sup.A -and .beta..sup.S -Globin Genes," DNA 3:1 (1984).
A.B. Studencki et al., "Discrimination among the Human .beta..sup.A, .beta..sup.SB, and .beta..sup.C - Globin Genes Using Allele-Specific Olignoucleotide Hybridization Probes," Am. J. Human Genetics 37:42 (1985).
Harrington, J.J. and Lieber, "Functional domains within FEN-1 and RAD2 define a family of structure-specific endonucleases: implications for nucleotide excision repair," Genes and Develop. 8:1344 (1994).
Murante, R.S., et al., "The Calf 5'- to 3'-Exonuclease Is Also an Endonuclease with Both Activities Dependent on Primers Annealed Upstream of the Point of Cleavage," J. Biol. Chem. 269:1191 (1994).
F.C. Lawyer et al., "Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene From Thermus aquaticus," J. Biol. Chem. 264:6427 (1989).
A.A. Akhmetzjanov and V.A. Vakhitov, "Molecular Cloning and Nucleotide Sequence of the DNA Polymerase Gene from Thermus flavus," Nucl. Acids Res. 20:5839 (1992).
P. Setlow et al., "Deoxyribonucle

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