Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1992-09-21
1993-10-05
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 71, 435 72, 435 721, 435 29, 435 34, 435240206, 436501, 436811, 436827, 530396, C12Q 170, G01N 3300
Patent
active
052504107
ABSTRACT:
A method for the rapid identification and differentiation of HSV-1 and/or HSV-2 with fluorescein-conjugated Helix pomatia lectin. Clinical specimens are processed, with centrifugation, in pre-prepared shell vials of cell culture, with subsequent supplementation and 20 hour incubation (approximately) at 37.degree. C. Coverslips of the shell vials are then fixed, air dried, overlaid with fluorescein-conjugated Helix pomatia lectin, washed, dried, mounted and examined under a microscope equipped with an ultraviolet light source. The identification of fluorescence confirms herpes virus infection. Granular fluorescent patterns confirm presence of HSV-2 serotype and diffuse fluorescent patterns confirm presence of HSV-1 serotype.
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Allegheny-Singer Research Institute
Kepplinger Esther L.
Spiegel Carol A.
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