Rapid detection of analytes with receptors immobilized on solubl

Details Rapid detection of analytes with receptors immobilized on solubl Rapid detection of analytes with receptors immobilized on solubl

1994-04-13

1999-04-27

Ceperley, Mary E.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

435 793, 435 794, 436500, 436512, 436530, 436823, 5303911, G01N 33552

Patent

active

058980059

ABSTRACT:
Methods are provided for conducting specific binding assays to determine the concentration or presence of at least one analyte in a sample. Dendrimer-reagent preparations with particular analyte specificities are mixed in solution with a sample to form dendrimer-reagent-sample complexes. The complexes are then immobilized on a solid phase. Immobilization is facilitated by coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

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Roberts, J.C. et al., Using Starburst Dendrimers as Linker Molecules to Radiolabel Antibodies, Bioconjug. Chemistry 1: 305-308 (1990).

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