Rapid detection method of Mycobacterium bovis by fluorescence po

Details Rapid detection method of Mycobacterium bovis by fluorescence po Rapid detection method of Mycobacterium bovis by fluorescence po

1997-06-27

2000-08-29

Duffy, Patricia A.

Chemistry: analytical and immunological testing

Involving immune complex formed in liquid phase

Signal modification or steric inhibition

436546, 436172, 530350, 530402, 530403, 530820, G01N 33542, C07K 100

Patent

active

061107506

ABSTRACT:
An assay for the detection of antibodies to the MPB70 protein secreted by Mycobacterium bovis utilizes a tracer consisting of the MPB70 protein conjugated to a fluorophore such as fluorescein. Upon mixing with the antibodies specific for MPB70 protein contained in the serum of an animal infected with M. bovis, the bound tracer exhibits an increase in fluorescence polarization, detectable in an instrument.

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