Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1993-03-12
1996-05-21
Czaja, Donald E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 435 29, 435 38, 435968, 4352521, 43525233, C12Q 102, C12Q 118, C12N 112
Patent
active
055188940
ABSTRACT:
A rapid presence-absence method for determining if fecal coliform cells are present in a sample. Portions of the original sample are filtered and retained upon microporous filters which are placed in incubation containers having an actuating medium containing a fluorogenic substrate. The samples are incubated for predetermined durations of from about twenty minutes to six hours. After adjusting the pH is incubation to an alkaline level, the containers are irradiated and the fluorescent light emitted is measured. The measured values are adjusted for background fluorescence and corrected for extraneous sources of fluorescence. It is concluded that fecal cells are present in the original sample (i.e., at least one fecal coliform cell per 100 milliliters) when the corrected fluorescence values are positive and meet certain predetermined criteria.
REFERENCES:
patent: 3928139 (1975-12-01), Dorn
patent: 4242447 (1980-12-01), Findl et al.
patent: 4289498 (1981-09-01), Baughman
patent: 4340671 (1982-07-01), Gibson
patent: 4591554 (1986-05-01), Koumura et al.
patent: 4693972 (1987-09-01), Mansour et al.
patent: 4777137 (1988-10-01), Lemonnier
patent: 4923804 (1990-05-01), Ley
patent: 4925789 (1990-05-01), Edberg
Maddacks, et al. Journal of Clinical Pathology vol. 28, No. 8, pp. 686-687, 1975.
W. R. Bailey, E. G. Scott (1966) Staining Formulae and Procedures, Chapter 37, in Diagnostic Microbiology, Mosby Co., St. Louis, pp. 318-319.
Standard Methods for the Examination of Water and Wastewater, Seventeen Ed., 1989, American Public Health Association, Washington, D.C., pp. 9-80 to 9-97.
G. C. Geesey, (Nov. 1987) Survival of Microorganisms in Low Nutrient Waters, Chapter 1, in Biological Fouling of Industrial Waste Water Systems: A Problem Solving Approach, Water Micro Associates, California, M. W. Mittleman and G. G. Geesey eds., pp. 1-23.
"ColiQuik" Coliform Detection Method of Hach Chemical Co., pp. 112, 113, 119 of Hach Catalog. (undated).
"Colifast" Coliform Monitoring Method by Palintest (Brochure), p. 20. (undated).
B. H. Olson, D. L. Clark, B. B. Milner, M. H. Stewart, and R. L. Wolfe, "Total Coliform Detection in Drinking Water: Comparison of Membrane Filtration with Colilert and Coliquik", Appl. Env. Micro., 57:1535-1539, May 1991.
Czaja Donald E.
Williams Jane
LandOfFree
Rapid coliform detection system does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rapid coliform detection system, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid coliform detection system will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2037268