Rapid C-ELISA process and related compositions

Details Rapid C-ELISA process and related compositions Rapid C-ELISA process and related compositions

2009-11-25

2010-10-19

Cheu, Jacob (Department: 1641)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S007200, C436S506000, C436S513000

Reexamination Certificate

active

07816092

ABSTRACT:
The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

REFERENCES:
patent: 6498016 (2002-12-01), Nahar et al.
patent: 6656715 (2003-12-01), Vreeland
patent: 2007/0134256 (2007-06-01), Lai et al.
Takahashi et al. (J. Pesticide Sci 1998 vol. 23, p. 386-391).
E. Engvall et al., Enzyme-linked immunosorbent assay (ELISA) Quantitative assay of immunoglobulin G, Immunochemistry (1971) vol. 8, No. 9, pp. 871-874.
J. Y. Douillard et al., Enzyme-linked immunosorbent assay for screening monoclonal antibody production using enzyme-labeled second antibody, Methods in Enzymology, (1983) vol. 92, pp. 168-174.
H. Hjelm et al., Immunologically active and structurally similar fragments of protein A fromStaphylococcus aureus, Eur. J. Biochem. (1975) vol. 57, No. 2, pp. 395-403.
W. Van Raamsdonk et al., Detection of antigens and antibodies by an immuno-peroxidase method applied on thin longitudinal sections of SDS-polyacrylamide gels, J. Immunol. Methods. (1977) vol. 17, pp. 337-348.
W. N. Burnette, “Western blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radioiodinated protein A. Analytical Biochemistry, (1981) vol. 112, No. 2, pp. 195-203.
R. Hawkes et al., A dot-immunobinding assay for monoclonal and other antibodies, Analytical Biochemistry. (1982) vol. 119, pp. 142-147.
L. Björck et al., Purification and some properties of streptococcal protein G, a novel IgG-binding reagent, J. Immunol. (1984) vol. 133, No. 2, pp. 969-974.
D.M. Boorsma, Direct immunoenzyme double staining applicable for monoclonal antibodies, Histochemistry (1984) vol. 80, No. 2, pp. 103-106.
K. Osther et al., The quick western blot, a novel transportable 50-minute HIV-1 antibody test, Transplantation (1989) vol. 47, No. 5, pp. 834-838.
K.X. Jacobsen et al., Vibration enhancement of slide-mounted immunofluorescence staining, J. Neurosci. Methods. (2004) vol. 137, pp. 71-77.

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