Rapid assaying method for guanase

Details Rapid assaying method for guanase Rapid assaying method for guanase

1982-09-27

1985-10-29

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 25, 435 27, 435 28, C12Q 134, C12Q 126, C12Q 128

Patent

active

045500762

ABSTRACT:
The guanase activity in body fluids such as blood serum can be rapidly and accurately assayed by (I) decomposing guanine with the guanase in the specimen to xanthine and ammonia at pH 7-9, preferably at pH 8, (II) decomposing the xanthine formed by former step I with xanthine oxidase to uric acid and hydrogen peroxide, (III) reacting the reactant solution of the former step II with 3-methyl-2-benzothiazolinonehydrazone, an aniline derivative such as N,N-di-lower-alkylaniline and peroxidase, and finally measuring the optical absorption of the reactant solution of the step III at 570-600 nm. The all steps can be completed within 15 minutes. Therefore, this assay is adoptable for automatic assay of guanase on usual clinically available automatic analyzers.

REFERENCES:
Slowiaczek et al., Anal. Biochem., 125: 6-12, (1982).
Kato et al., Chemical Abstracts, 92: 159394c, p. 204, (1980).
Sugiura et al., Chemical Abstracts, 94: 169969z, p. 288, (1981).
Ando et al., Chemical Abstracts, 98: 156866w, p. 183, (1983).
van Bennekom et al., J. Clin. Chem. Clin. Biochem., 16: 245-248, (1978).
Roush et al., Arch. Biochem., vol. 29, pp. 124-129, (1950).
Hue et al., Clin. Chem., vol. 11, pp. 708-715, (1965).
Kalcker, J. Biol. Chem., vol. 167, pp. 429-459.

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