Rapid assay for testing overall oxidative stress

Details Rapid assay for testing overall oxidative stress Rapid assay for testing overall oxidative stress

2005-02-08

2005-02-08

Alexander, Lyle A. (Department: 1743)

Chemistry: analytical and immunological testing

Blood gas

C436S008000, C436S016000, C436S063000, C436S062000, C436S084000, C436S161000, C436S162000, C436S164000, C436S166000, C436S169000, C436S174000, C436S810000, C436S811000, C436S815000, C422S067000, C422S051000, C422S068100, C422S079000, C422S082050

Reexamination Certificate

active

06852541

ABSTRACT:
The invention relates to an assay for testing oxidative stress of a subject by measurement of oxidants in biological fluids such as urine, plasma, bioreactor medium and respiratory aspirants. There is provided a method of determining oxidative stress in a mammalian subject. The method comprises: obtaining a sample of a biological fluid from the subject; mixing the biological fluid with a ferrous reaction reagent; incubating the biological fluid and the reaction reagent; and detecting a colored reaction product.There is further provided a ferrous reaction reagent suitable for use in assaying oxidative stress, said reaction reagent comprising 2-deoxyglucose, TBA, EDTA, and ferrous sulfate, and being substantially free of ascorbic acid.

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Long et al.,Biochem. Biophys. Res. Comm.,262:605-609 (1999).
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