Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-04-08
2000-06-20
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 721, C12Q 168, G01N 3353
Patent
active
06077665&
ABSTRACT:
A rapid assay for infection in immunodeficient patients such as neonates or immunocompromised patients (e.g. HIV or transplant patients) allows diagnosis at initial evaluation, such that antibiotic treatment and confinement to an intensive care unit can be avoided for uninfected patients. The assay can be used for detecting bacterial, viral, or fungal colonization of the blood stream, cerebrospinal fluid (CSF), or urinary tract. The method is particularly useful for sepsis diagnosis. Polymorphonuclear leukocyte (PMN, neutrophil) CD11b (Mac-1, CR3) levels are measured by flow cytometry or laser scanning microscopy in low volume (0.1 ml) whole blood samples. A dual-laser FACS identifies neutrophils by FITC-conjugated anti-CD15 fluorescent antibodies, and identifies surface neutrophil CD11b marked with PE-conjugated anti-CD11b antibodies. Spontaneous upregulation of CD11b is prevented by handling samples at 4.degree. C. or adding a stabilizing compound such as anti-CD14 antibody or adenosine to the samples. A kit comprises calibration markers such as CD11b-coated microspheres for calibrating the device used for analysis according to diagnosis threshold(s).
REFERENCES:
G. Escobar et al., Early discontinuation of antibiotic treatment in newborns admitted to rule out sepsis: a decision rule, Pediatr Infect Dis J. , vol. 13, No. 10, 1994:13:860-6.
W. Lynn et al., lipopolysaccharide-induced stimulation of CD11b/CD18 expression on neutrophils, Journal of Immunology, vol. 147, 3072-3079, No. 9, 1991.
H. Repo et al.,flow cytometric determination of CD11b upregulation in vivo, Journal of Immunological Methods, 164 (1993) 193-202.
H. Fleit, Fc and complement receptor (CR1 and CR3) expression on neonatal human poly-morphonuclear leukocytes, Biol Neonate, 1989; 55:156-163.
R. Lin, Relationships between plasma cytokine concentrations and leukocyte functional antigen expression in patients with sepsis, Critical Care Medicine, vol. 22, No. 10, 1994, 1595-1601.
M. Radetsky, The laboratory evaluation of newborn sepsis, Current Science, 1995, 8:191-199.
D. Anderson, Neonatal neutrophil dysfunction, American Journal of Pediatric Hematology/Oncology, 11(2):224-226, 1989.
N. Abughali, Deficient total cell content of CR3 (CD11b) in neonatal neutrophils, Blood, vol. 83 No. 4, Feb. 15, 1994, pp. 1086-1092.
R. Rivers, The expression of surface tissue factor apoprotein by blood monocytes in the course of infections in early infancy, Pediatric Research, vol. 31, No. 6, 1992, pp. 567-573.
D. Gendrel et al., Procalcitonin as a marker for the early diagnosis of neonatal infection, Journal of Pediatrics, vol. 128, No. 4, Apr. 1996, pp. 570-573.
B. Davis et al., Neutrophil CD64 expression: potential diagnostic indicator of acute inflammationn and therapeutic monitor of interferon-.gamma. therapy, Lab Hematology, 1:3-12.
Spear et al. J. Pediatrics (Jun. 1995) 126: 982-5.
Abughali et al. Blood (Feb. 15, 1994) 83(4): 1086-1092.
Lin et al. Chest (1993) 104(3): 847-53.
ESJM de Bont et al. ACTA Pediactrics (1994) 83: 696-9.
Buck et al. Pediatrics (1994) 93: 54-8.
Gendrel et al. (Apr. 1996) J. Pediatrics 128(4): 570-573.
Weirich et al. J. Pediatrics (Mar. 1998) 132(3): 445-451.
Elbim et al. J. Cardio. Pharmacol. 25(Sup. 2)S66:S70 1995.
Lanza et al. Int.J.Clin Lab Res. 23: 146-150 (1993).
Rebuck et al. Clin Exp. Immunol. (1995) 101: 183-189.
Smith et al. Ped. Res. (1991) 30(4): 355-361.
Benitz William E.
Herzenberg Leonard A.
Herzenberg Leonore A.
Maldonado Yvonne
Rabin Ronald L.
Arthur Lisa B.
Popovici Andrei
Sherwood Pamela
The Board of Trustees of the Leland Stanford Junior University
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