Rapid assay for functional human .alpha..sub.1 -proteinase inhib

Details Rapid assay for functional human .alpha..sub.1 -proteinase inhib Rapid assay for functional human .alpha..sub.1 -proteinase inhib

1989-01-30

1991-12-17

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 13, 435 24, 435 19, 530380, 530392, 530829, 530830, C12Q 137

Patent

active

050734873

ABSTRACT:
A simple, rapid and reliable assay for measuring functional enzyme inhibitor levels in body fluids and tissues, especially functional .alpha..sub.1 -proteinase inhibitor levels in human plasma or serum. .alpha..sub.2 -Macroglobulin is first inactivated, then porcine pancreatic elastase incubated with the samples to form a complex between the elastase and the functional .alpha..sub.1 -PI. Deficient individuals are detected by the presence of a color change following addition of substrate. If desirable, residual enzyme activity can then be calculated and the .alpha..sub.1 -PI levels present in the original sample determined. The method provides a means for early screening of individuals with a genetic deficiency in circulating levels of .alpha..sub.1 -PI, thereby facilitating treatment and prevention of familial emphysema.

REFERENCES:
patent: 4493891 (1985-01-01), Travis
Bjork et al. (1985), Biochemistry, vol. 24, ppo. 2653-2660.
Feldman, et al., Proc. Natl. Acad. Sci., 82, 5700-5704 (Sep. 1985).
Gaillard et al., J. Clin. Chem. Biochem., 26, 167-172 (1987).
Hubbard et al., JAMA, 1259-64 (1988).

Affiliated with

Also associated with

Rating

Rapid assay for functional human .alpha..sub.1 -proteinase inhib does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Rapid assay for functional human .alpha..sub.1 -proteinase inhib, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid assay for functional human .alpha..sub.1 -proteinase inhib will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-835200