Rapid and sensitive detection of herpes simplex virus

Details Rapid and sensitive detection of herpes simplex virus Rapid and sensitive detection of herpes simplex virus

1996-02-13

1998-12-08

Myers, Carla J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435912, 435810, 536 2432, 536 2433, 935 8, 935 11, 935 78, C12Q 168, C12P 1934, C07H 2104

Patent

active

058467068

ABSTRACT:
The present invention relates to novel compositions comprising herpesvirus-specific oligonucleotides which are useful as primers to amplify particular regions of the genome either herpes simplex virus or cytomegalovirus during enzymatic nucleic acid amplification. The invention also provides a rapid, sensitive and specific method for the detection of the respective herpesvirus which may be present in a clinical specimen, using the herpesvirus-specific primers and enzymatic nucleic acid amplification; hybridization of amplified target sequences, if present, with one or more herpesvirus-specific oligonucleotide probes which are labeled with a detectable moiety; and detection of the detectable moiety of labeled oligonucleotide probe hybridized to amplified target sequences of herpesvirus DNA.

REFERENCES:
patent: 5514577 (1996-05-01), Draper
Shimizu et al. Molecular and Cellular Probes (1994) 8:193-198.

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