Rapid and sensitive assay for homocysteine

Details Rapid and sensitive assay for homocysteine Rapid and sensitive assay for homocysteine

1999-06-14

2000-04-04

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 26, 435 15, 435975, 435 4, C12Q 126, C12Q 132, C12Q 148, C12Q 100

Patent

active

060460172

ABSTRACT:
Methods and kits for assaying homocysteine in biological samples utilizing enzymatic reactions are provided. One method utilizes the enzymes methionine synthase and serine hydroxymethyltransferase sequentially to produce .sup.3 H.sub.2 O in proportion to the amount of hCys in the original sample. Another method utilizes the enzyme methionine synthase and then the enzymes serine hydroxymethyltransferase, methylene tetrahydrofolate dehydrogenase and formyl tetrahydrofolate dehydrogenase, to produce NADPH in proportion to the amount of hCys in the original sample.

REFERENCES:
Steen et al, "Prenatal Diagnosis", vol. 18 (6), p545 (Abstract), Jun. 1998.
Sturman et al; J. Neurochem., vol. 27 (2), p425 (Abstract), 1976.

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