Rapid and high precision centroiding method and system for...

Image analysis – Pattern recognition – Feature extraction

Reexamination Certificate

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C382S288000, C382S103000

Reexamination Certificate

active

08068673

ABSTRACT:
The disclosure relates to a rapid and high precision centroiding method for spots image comprising the following steps. First, the method requires convoluting the gray value of a pixel with a Gaussian filter and judging whether the result thereof exceeds a predetermined threshold. If the value exceeds the predetermined threshold, the method requires marking the current pixel with a flag to identify which spot it belongs to, and then accumulating the product of the gray value and a coordinate value of the same spot; and at the same time, accumulating the gray value of the same spot; and saving the results of the accumulation respectively. If the gray value of the pixel does not exceed the predetermined threshold, the method requires marking the current pixel as a background flag. After all pixels of the image has been disposed of completely, the method includes calculating a quotient of the accumulations of the product of the gray value and the coordinate value and the accumulations of the gray value, and outputting the quotients. At the same time, a centroiding system for spots image is also presented.

REFERENCES:
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patent: 6650766 (2003-11-01), Rogers et al.
patent: 2001/0008562 (2001-07-01), Rogers et al.
patent: 2003/0071819 (2003-04-01), Kondo et al.
patent: 2003/0086063 (2003-05-01), Williams et al.
patent: 2004/0001074 (2004-01-01), Oyaizu
patent: 2005/0232483 (2005-10-01), Kato et al.
patent: 2006/0103663 (2006-05-01), Collodi
patent: 2007/0159627 (2007-07-01), Johnson

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