Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2005-08-30
2005-08-30
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S455000, C435S320100
Reexamination Certificate
active
06936470
ABSTRACT:
A method for cloning a nucleic acid fragment into a vector by flanking the fragment with first and second adapter sequences, and contacting the fragment with the vector having sequences homologous to the first and second adapter sequences under conditions such that the nucleic acid fragment is incorporated into the vector by homologous recombination in vivo in a host cell. Additionally, a method for selecting for a successful transformation of a vector by a nucleic acid insert. Also, systems for cloning a nucleic acid fragment into a vector without at least one of a restriction enzyme, a ligase, a gyrase, a single stranded DNA binding protein, or other DNA modifying enzymes. Further, a kit for cloning a nucleic acid fragment into a vector.
REFERENCES:
patent: 4897355 (1990-01-01), Eppstein et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5459127 (1995-10-01), Felgner et al.
patent: 5561053 (1996-10-01), Crowley
patent: 5612180 (1997-03-01), Brown et al.
patent: 5621080 (1997-04-01), Lin
patent: 5736365 (1998-04-01), Walker et al.
patent: 6063571 (2000-05-01), Uhlmann et al.
patent: 6063604 (2000-05-01), Wick et al.
patent: 6165720 (2000-12-01), Felgner et al.
patent: 6221588 (2001-04-01), Bradshaw et al.
patent: 6280977 (2001-08-01), Liang et al.
patent: 6509156 (2003-01-01), Stewart et al.
Zhang et al. A new logic for DNa engineering using recimbinaiton inEscgerucgua coliNature Genetics vol. 20 1998.
Cassata, Giuseppe, et al., “Rapid expression of Caenorhabditis elegans homeobox open reading frames using a two-step polymerase chain reaction promoter gfp reporter construction technique”, Gene, An International Journal on Genes and Genomes, vol. 212, pp. 127-135 (1998).
Goodchild, John. “Conjugates of Oligonucleotides and Modified Oligonucleotides: A Review of Their Synthesis and Properties”, Bioconjugate Chemistry, vol. 1, No. 3, pp. 166-187 (May/Jun. 1990).
Prodromou, Chrisostomos, et al., “PROTOCOL, Recursive PCR: a novel technique for total gene synthesis”, Protein Engineering, vol. 5, No. 8, pp. 827-829 (1992).
Smith et al., “Genetic footprinting: A genomic strategy for determining a gene's function given its sequence,”Proc. Natl. Acad. Sci. USA, 92: 6479-6483 (1995).
Vos et al., “AFLP: a new technique for DNA fingerprinting,”Nucleic Acids Research, 23: 4407-4414 (1995).
Almarsson, et al. “Peptide Nucleic Acid (PNA) Conformation and Polymorphism in PNA-DNA and PNA-RNA Hybrids.”Proc. Natl. Acad. Sci. USA, 90 9542-9546 (1993).
Benlin, et al. “Enhanced Peptide Nucleic Acid Binding to Supercoiled DNA Possible Implications for DNA “Breathing” Dynamics,”Biochem., 35 8853-8859 (1996).
Bradshaw, et al. “A New Vector for Recombination-based Cloning of Large DNA Fragments from Yeast Artificial Chromosomes,”Nucleic Acids Research,23(23) 4850-4856 (1995).
Clark, J M. “Novel Non-templated Nucleotide Addition Reactions Catalyzed by Procaryotic and Eucaryolic DNA Polymerases,”Nucleic Acids Research,16(20) 9677-9686 (1988).
Demidov,et al. “Kinetics and Mechanism of Polyamide (“Peptide”) Nucleic Acid Binding to Duplex DNA.”Proc. Natl Acad Sci. USA, 92 2637-2641 (1995).
Demidov,et al. “Stability of Peptide Nucleic Acids in Human Serum and Cellular Extracts,”Biochem Pharmacology, 48(6) 1310-1313 (1994).
Egholm, et al. “Efficient pH-Independent Sequence-Specific DNA Binding by Pseudoisocytosine-Containing bis-PNA,”Nucleic Acids Research,23(2) 217-222 (1995).
Egholm, et al. “PHA Hybridizes to Complementary Oligonucleotides Obeying the Watson-Crick Hydrogen-bonding Rules,”Nature365 566-568 (1993).
Egholm, et al. “Recognition of Guanine and Adenine in DNA by Cytosine and Thymine Containing Peptide Nucleic Acids (PNA),”J Am Chem. Soc. 114 9677-9678 (1992).
Fakhfahk, et al. “Cell-free Cloning and Biolistic Inoculation of an infectious cDNA of Potato Virus Y,”J. of Gen. Virology, 77 519-523 (1996).
Felgner, et al. “Nomenclature for Synthetic Gene Delivery Systems,”Human Gene Therapy, 8 511-512 (1997).
Griffith, et al. “Single and Bis Peptide Nucleic Acids as Triplexing Agents Binding and Stoichiometry,”J. Am. Chem. Soc. 117 831-832 (1995).
Higuchi, R. “Recombinant PCT,”PCR Protocols: A Guide to methods and ApplicationsNew York Academic Press p 177-183 (1990).
Ido, et al. “Contruction of T-Tailed Vectors Derived from a pUC Plasmid a Rapid System for Direct Dloning of Unmodified PCR Products,”Biosci. Biotech. Biochem., 61(10) 1766-1767 (1997).
Li, et al. “Delivery of a PCR Amplified DNA Fragment into Cells A Model for Using Synthetic Genes for Gene Therapy,”Gene Therapy, 4 449-454 (19997).
Nielsen, et al, “Sequence-Selective Recognition of DNA by Strand Displacement with a Thymine-Substituted Polyamide,”Science, 254 1497-1500 (1991).
Oliner, et al., “In vivo Cloning of PCR Products inE. coli,” Nucleic Acids Research, 21(22) 5192-5197 (1993).
Seed, B., “Purification of Genomic Sequences from Bacteriophage Libraries by Recombination and Selection in vivo,”Nucleic Acids Research, 11(8) 2427-2445 (1983).
Sykes, et al., “Linear Expression Elements, A Rapid, in vivo. Method to Screen for Gene Functions,”Nature Biotechnology, 17 355-359 (1999).
Zhang, et al., “A New Logic for DNA Engineering Using Recombination inEschenichia coli,” Nature Genetics, 20 123-128 (1998).
Zhang, et al., “DNA Cloning by Homologous Recombination inEschenichia coli,” Nature Biotechnology, 18 1314-1317 (2000).
Chen Shizhong
Felgner Philip L.
Liang Xiaowu
Teng Andy
Xia Dongyuan
Gene Therapy Systems, Inc.
Katcheves Konstantina
Ketter James
LandOfFree
Rapid and enzymeless cloning of nucleic acid fragments does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rapid and enzymeless cloning of nucleic acid fragments, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid and enzymeless cloning of nucleic acid fragments will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3479116