Rapid and efficient capture of DNA from sample without using...

Details Rapid and efficient capture of DNA from sample without using... Rapid and efficient capture of DNA from sample without using...

2006-12-20

2009-11-10

Kim, Young J (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200

Reexamination Certificate

active

07615346

ABSTRACT:
Nucleic acids can be made available for amplification or other treatment after admixture of a sample with specific weakly basic polymers to form a precipitate with the nucleic acids at acidic pH. After removing non-precipitated materials, the pH is then made basic, thereby releasing the nucleic acids from the polymer. This method for preparing specimen samples is simple and quite rapid, and the released nucleic acids can be further treated in hybridization assays or amplification procedures. No surfactant or other cell lysing reagents are employed. The weakly basic polymers are water-soluble and cationic at acidic pH, but neutral in charge at basic pH.

REFERENCES:
patent: 4089747 (1978-05-01), Bruschi
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4713326 (1987-12-01), Dattagupta et al.
patent: 4795698 (1989-01-01), Owen et al.
patent: 4889818 (1989-12-01), Gelfand et al.
patent: 4914210 (1990-04-01), Levenson et al.
patent: 4920061 (1990-04-01), Poynton et al.
patent: 4962029 (1990-10-01), Levenson et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4994373 (1991-02-01), Stavrianopoulos et al.
patent: 4997772 (1991-03-01), Sutton et al.
patent: 5118801 (1992-06-01), Lizardi et al.
patent: 5147777 (1992-09-01), Sutton et al.
patent: 5155166 (1992-10-01), Danielson et al.
patent: 5173260 (1992-12-01), Zander et al.
patent: 5229297 (1993-07-01), Schnipelsky et al.
patent: 5231015 (1993-07-01), Cummins et al.
patent: 5312728 (1994-05-01), Lizardi et al.
patent: 5334499 (1994-08-01), Burdick et al.
patent: 5338671 (1994-08-01), Scalice et al.
patent: 5380489 (1995-01-01), Sutton et al.
patent: 5434270 (1995-07-01), Ponticello et al.
patent: 5523368 (1996-06-01), Ponticello et al.
patent: 5582988 (1996-12-01), Backus et al.
patent: 5587287 (1996-12-01), Scalice et al.
patent: 5622822 (1997-04-01), Ekeze et al.
patent: 5683877 (1997-11-01), Lu-Chang et al.
patent: 5702884 (1997-12-01), Ekeze et al.
patent: 5925517 (1999-07-01), Tyagi et al.
patent: 2158485 (1996-03-01), None
patent: 0 070 687 (1985-10-01), None
patent: 0 308 236 (1989-03-01), None
patent: 0 320 308 (1989-06-01), None
patent: 0 408 738 (1990-08-01), None
patent: 0 393 744 (1990-10-01), None
patent: 0 428 197 (1991-05-01), None
patent: 0 439 182 (1991-07-01), None
patent: 0 439 222 (1991-07-01), None
patent: 0 487 218 (1992-05-01), None
patent: 0 512 334 (1992-11-01), None
patent: 0 707 077 (1996-04-01), None
patent: 0 953 635 (1999-11-01), None
patent: 0 871 767 (2002-03-01), None
patent: 2 327 497 (1999-01-01), None
patent: WO 89/06691 (1989-07-01), None
patent: WO 91/12342 (1991-08-01), None
patent: WO 92/16659 (1992-10-01), None
patent: WO 95/16792 (1995-06-01), None
patent: WO 96/15262 (1996-05-01), None
patent: WO 96/32500 (1996-10-01), None
patent: WO 97/34015 (1997-09-01), None
patent: WO 99/04037 (1999-01-01), None
patent: WO 01/49877 (2001-07-01), None
Gwynne et al., “Drug Discovery and Biotechnology Trends Genomes and Microbes: Resisting Drug Resistance,” online publication from www.sciencemag.org, publication date, May 9, 2003, pp. 1-8 (print out).
Manen et al., “A fully automatable enzymatic method for DNA extraction from plant tissues,” BMC Plant Biology, vol. 5, No. 23, pp. 1-9.
Mulcahy, H.E. et al., “A Prospective Study of K-ras Mutations in the Plasma of Pancreatic Cancer Patients”, Clinical Cancer Research, vol. 4, pp. 271-275, Feb. 1998.
Lo, Y.M. et al., “Quantitative Analysis of Fetal DNA in Maternal Plasma and Serum: Implications for Noninvasive Prenatal Diagnosis”, Am. J. Hum. Genet. 62:768-775, 1998.
Kopreski, M.S. et al., “Detection of mutant K-ras DNA in plasma or serum of patients with colorectal cancer”, British Jouurnal of Cancer, 76(101):1293-1299, 1997.
Agrawal, Sudhir, et al., “Efficient Methods for Attaching Non-Radioactive Labels to the 5' Ends of Synthetic Oligodeoxyribonucleotides”,Nucleic Acid Res.,1986, 14(15):6227-45.
Ahnen, Dennis J., et al., “Ki-rasMutation and p53 Overexpression Predict the Clinical Behavior of Colorectal Cancer: a Southwest Oncology Group Study”,Cancer Research, Mar. 15, 1998, 58:1149-1158.
Andreyev, H. Jervoise, et al., “Kirsten ras Mutations in Patients with Colorectal Cancer: the Multicenter ‘Rascal’ Study”,Journal of the National Cancer Institute, May 6, 1998, 90(9);675-684.
Anker, Philippe, et al., “K-rasMutations Are Found in DNA Extracted From the Plasma of Patients With Colorectal Cancer”Gastroenterology, 1997, 112(4):1114-1120.
Aoki, Takahisa, et al., “APC and p53 Mutations in de Novo Colorectal Adenocarcinomas”,Human Mutation, 1994, 3(4):342-346.
Bos, Johannes L., et al., “Three Different Mutations in Codon 61 of the Human N-rasGene Detected by Synthetic Oligonucleotide Hybridization”,Nucleic Acids Research, 1984, 12(23):9155-9163.
Bos, Johannes L., et al., “Prevalence ofrasGene Mutations in Human Colorectal Cancers”,Nature, May 1987, 327(6120):293-297.
Bos, Johannes L., et al., “RasOncogenes in Hematopoietic Malignancies”,Hematologic Pathology, 1988, 2(2):55-63.
Bos, Johannes L., et al., “TherasGene Family and Human Carcinogenesis”,Mutation Research, 1988, 195:255-271.
Bos, Johannes L., et al., “rasOncogenes in Human Cancer: A Review”,Cancer Research, Sep. 1, 1989, 49:4682-4689.
Calaluce, Robert, M.D., et al., “Micrometastasis in Colorectal Carcinoma: A Review”,Journal of Surgical Oncology, 1998, 67:194-202.
Capon, Daniel J., et al., “Activation of Ki-ras2 Gene in Human Colon and Lung Carcinomas by Two Different Point Mutations”,Nature, Aug. 1983, 304:507-513.
Castells, Antoni, et al., “K-rasMutations in DNA Extracted From the Plasma of Patients with Pancreatic Carcinoma: Diagnostic Utility and Prognostic Significance”,Journal of Clinical Oncology, Feb. 1999, 17(2):578-584.
Chen, Xu Qi, et al., “Microsatellite Alterations in Plasma DNA of Small Cell Lung Cancer Patients”,Nature Medicine, Sep. 1996, 2(9):1033-1035.
Cohen, Justus B., et al., “A Point Mutation in the Last Intron Responsible for Increased Expression and Transforming Activity of the c-Ha-rasOncogene”,Nature, Jul. 1988, 334:119-124.
Dukes, Cuthbert E., “The Classification of Cancer of the Rectum”,J. PathoL Bacteriol., 1932, 35:323-332.
Ferguson, Wilfred. J., et al., “Hydrogen Ion Buffers for Biological Research”,Analytical Biochemistry, 1980, 104:300-310.
Findlay, John B., et al., Automated Closed-Vessel System for in Vitro Diagnostics Based on Polymerase Chain Reaction,Clin. Chem., 1993, 39(9):1927-1933.
Fournie, Gilbert J., et al., “Plasma DNA as a Marker of Cancerous Cell Death. Investigations in Patients Suffering From Lung Cancer and in Nude Mice Bearing Human Tumours”,Cancer Letters, 1995, 91:221-227.
Fuery, Caroline J., et al., “Detection of Rare Mutant Alleles by Restriction Endonuclease-mediated Selective-PCR: Assay Design and Optimization”,Clinical Chemistry, 2000, 46(5):620-624.
Good, Norman E., et al., “Hydrogen Ion Buffers for Biological Research”,Biochemistry, Feb. 1966, 5(2):467-477.
Good, Norman E. and Izawa, S., “Hydrogen Ion Buffers”,Methods in Enzymology, Photosynthesis and Nitrogen Fixation, 1972, Anthony San Pietro, Ed., 24(Part B):53-68, Academic Press, New York and London.
Gross-Bellard, Maria, et al., “Isolation of High-Molecular-Weight DNA from Mammalian Cells”,Eur. J. Biochem., 1973, 36(1):32-38.
Haliassos, a., et al., “H-rasOncogene Mutations in the Urine of Patients with Bladder Tumors: Description of a Non-Invasive Method for the Detection of Neoplasia”,International Journal of Oncology, 1992, 1:731-734.
Hayashi, Naoko, et al., “Genetic Diagnosis Identifies Adult Lymph Node Metastases Undetectable by the Histopathological Method”,Cancer Research, 1994, 54:3853-3856.
Hayashi, Naoko, et al., “G

Affiliated with

Also associated with

Rating

Rapid and efficient capture of DNA from sample without using... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Rapid and efficient capture of DNA from sample without using..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid and efficient capture of DNA from sample without using... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4092986