Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2007-07-03
2007-07-03
Weber, Jon (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
Reexamination Certificate
active
10635164
ABSTRACT:
A method for in situ detection of viable pathogenic bacteria in a selective medium by measuring cathodic peak current of oxygen on cyclic voltammograms during bacterial proliferation with an electrochemical voltammetric analyzer. The rapid oxygen consumption at a time during the growth of bacteria resulted in a sharp decline of the cathodic peak current curves. The detection times (threshold values) obtained from the cathodic peak current curve were inversely related to the concentrations of the pathogenic bacteria in the medium. This method for detection of pathogenic bacteria is more sensitive than nucleic acid-based polymerase chain reaction (PCR) methods and any of antibody-based methods such as enzyme-linked immunosorbent assay (ELISA) technology, electrochemical immunoassays, immunosensors, and it has a sensitivity similar to conventional culture methods and impedimetric methods but is more rapid than both of them. A calibration curve was obtained by plotting initial cell concentrations (CFU/ml) determined by conventional plate counting, as a function of the detection time.
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Li Yanbin
Ruan Chuanmin
Yang Liju
Fahrlander Jill A.
Michael & Best & Friedrich LLP
Srivastava Kailash C.
The Board of Trustees of the University of Arkansas
Weber Jon
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