Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids
Patent
1998-05-20
2000-02-01
Warden, Sr., Robert J.
Chemistry: analytical and immunological testing
Metal or metal containing
Present in biological fluids
436166, 436 84, G01N 3320, G01N 2178
Patent
active
060202041
ABSTRACT:
An inventive method and reagent for the colorimetric determination of nickel and cobalt is provided. The method and reagent uses dithiothreitol and a phosphate buffer to quantitatively detect nickel and cobalt at 465 nm and 485 nm, respectively. The method and reagent are accurate over a nickel concentration range of 1.76 to 35.2 ppm, and over a cobalt concentration range of 0.587 to 17.6 ppm. The invention is valid in protein solutions, requires no extractions and uses stable reagents. Common metals found in biological system do not interfere with the method.
REFERENCES:
patent: 5925570 (1999-07-01), Nishidate et al.
"Colorimetric and Spectrophotometric Analysis", Textbook of Quantitative Inorganic Analysis, pp. 794-795, Vogel 1961.
S. E. Allen, Chemical Analysis of Ecological Materials, pp. 327-329, (1974).
F. W. Sunderman, Jr., "Analytical Biochemistry of Nickel", Pure and Applied Chemistry, V 52, pp. 529-544 (1980).
"A highly sensitive extraction-photometric method for nickel with dithizone and phenanthroline", Talanta, vol. 16, pp. 412-414 (1969).
A.K. Majumdar, et al., "Chromotropic Acid Azo Dyes of the Pyridine Series", Talanta, vol. 13, pp. 821-828 (1966).
C.G. Perez, et al., "Spectrophotometric Determination of Nickel by Oxidation of the Nickel (II)--Nioxime Complex with Hexacyanofei Rate (III) in Sodium Hydroxide Medium", Analytica Chimica Acta. 87, pp. 233-237 (1976).
P. Koch, et al., "The Nickel-Catalysed Carbonylation of 2-Mercaptoethanol", Journal of Organometallic Chemistry, 81, pp. 111-114 (1974).
B. S. Snyder, et al., Mono-and Bi-nuclear Metal (II) Complexes of Ethane-1, 2-dithiolate: Preparation, Equilibrium and Redox Properties of [Ni(S.sub.2 C.sub.2 H.sub.4).sub.2 ].sup.2 --and [Ni(S.sub.2 C.sub.2 H.sub.4).sub.3 ].sup.2 --, and the Structures of Ni.sub.2 (S.sub.2 C.sub.2 H.sub.4).sub.3 .sup.2 --and [Pd(S.sub.2 C.sub.2 H.sub.4).sub.2 ].sup.2 --Aust J. Chem, 39, pp. 963-974 (1986).
S.K. Argra, et al., "The Ethylammonium Salt of the Nickel (II) Complex of Ethyldithiophosphonic Acid", Acta Cryst., B34, pp. 3355-3357 (1978).
M.W. Grant, et al., "The Lewis-Acidity of Square-Planar Nickel (II) Complexes. I A Kinetic and Thermodynamic Study of the Reaction of Bis(O,O-diethyl phosphorodithioato-S,S'-)nickel(II) with bidentate Nitrogen Donors", Aust. J. Chem., 29, pp. 749-758 (1976).
R.W. Burke, et al., "Procedures for the Determination of Arsenic, Copper, and Nickle by Molecular Absorption Spectrometry", Section V, Molecular Absorption Spectrometry, pp. 73-84.
"Nickel: Inorganic & Coordination Chemistry", Encylopedia of Inorganic Chemistry, vol. 5, pp. 2393-2395 & 2404-2405 (1994).
"Chapter 5: General Aspects of Trace Element Analysis", Trace Element Analysis, Vlado Valkovic, 1975, pp. 115-117.
H. Onishi, "Photomoetric Determination of Traces of Metals Part II B", Chemical Analysis, vol. III, pp. 122-150 (1989).
E.W. Abel, et al., "Cyclic Hexameric Mercaptides of Nickel", Journal of the Chemical Society, Sec. A, pp. 1377-1378 (1966).
G.A. Barclay et al., "Sulphur-Bridged Complexes Involving the Ligand 2,2'-Dimercaptodiethyl Sulphide", Aust. J. Chem., 21, pp. 2669-2674 (1968).
C. Watters, "A One-Step Biuret Assay for Protein the Presence of Detergent", Analytical Biochemistry, pp. 695-698 (1978).
R.E. Beyer, "A Rapid Biuret Assay for Protein of Whole Fatty Tissues", Analytical Biochemistry, 129, pp. 483-485 (1983).
M.W. Scoggins, "Ultraviolet Spectrophotometric Determination of Nickel", Analytical Chemistry, 42, No. 2, pp. 301-303 (1970).
D.P. Ambhore, et al., "Simultaneous Determination of Cobalt & Nickel with 3-thiobenzoyl-1-p-tolythiocarbamide", Indian Journal of Chemistry, vol. 25A, pp. 699-700 (Jul. 1986).
Chenoweth Matthew R.
DerVartanian Daniel V.
Snider Theresa T.
University of Georgia Research Foundation Inc.
Warden, Sr. Robert J.
LandOfFree
Rapid and accurate colorimetric determination of nickel and coba does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Rapid and accurate colorimetric determination of nickel and coba, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Rapid and accurate colorimetric determination of nickel and coba will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-936807