Rapid analysis of plural components

Details Rapid analysis of plural components Rapid analysis of plural components

1990-11-20

1992-12-29

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 4, 435 18, 435 21, 435 25, C12Q 154, C12Q 126, C12Q 100

Patent

active

051750880

ABSTRACT:
A new method of rapidly analyzing plural substances in the presence of biological catalyzers is disclosed. The method is practiced by way of the steps of injecting both pH buffer solution and specimen into a reaction cell, successively adding a plurality of enzymes to induce up-take reaction of dissolved oxygen, causing the plurality of substances to be subjected to selective oxidation in the stepwise manner, obtaining a stepdown curve of dissolved oxygen by automatically recording the oxidative process by means of a dissolved oxygen sensor, qualitatively determining each of the substances with reference to the kind of added enzymes and the order of their addition and quantitatively determining the same with reference to the extent of decrease in dissolved oxygen. Typically, oxidation of the substances is carried out by way of two or three or further more steps. The reaction cell for performing the method is equipped with a dissolved oxygen sensor on the one side wall and its upper portion is air-tightly closed with a plug through which a fine bore is formed for the purpose of supply of buffer solution, specimen and enzymes.

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