Rapid analysis of ethanol in body fluids

Details Rapid analysis of ethanol in body fluids Rapid analysis of ethanol in body fluids

1992-11-19

1994-03-01

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 27, 435 28, 435184, 435188, 435190, 435192, 435176, 422 56, 422 57, 422 69, C12Q 132, C12N 1114, C12N 996, C12N 3122

Patent

active

052906833

ABSTRACT:
An assay for directly and rapidly determining the alcohol concentration of a lower alcohol in a biological fluid without dilution, comprises a solid carrier. The carrier is impregnated with a solution having the following constituents mixed in effective amounts: a cross-linked stabilized enzyme for oxidizing the alcohol in the presence of a reducible compound to produce a corresponding aldehyde and a reduction product, a chromogen which reacts with the reduction product in the presence of an agent to produce a colored compound indicative of alcohol presence in the biological fluid, a competitive inhibitor of the enzyme, an agent for converting the chromogen to the colored compound, and a buffer to maintain the solution at a predetermined acidic pH.

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Majkic-Singh Analytica Chimica Acta, vol. 115, pp. 401-405.

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