Rapid amplification and detection of nucleic acids

Details Rapid amplification and detection of nucleic acids Rapid amplification and detection of nucleic acids

1993-12-22

1996-10-29

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 536 2433, 536 243, 536 2432, 935 77, 935 78, C12Q 170, C12Q 168, C07H 2104

Patent

active

055695825

ABSTRACT:
Disclosed herein are methods, primers, probes, and kits for the rapid amplification and detection of nucleic acids. The invention provides improved methods for amplifying small amounts of nucleic acid in a sample in which the amplification steps are conducted at the same temperature, or alternatively, at two different temperatures. The invention also provides improved methods for detecting the amplified nucleic acid in which the detection signal is boosted. Related probes and test kits are also provided. The invention is particularly useful in the detection of HIV-1.

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