Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-04-09
1994-06-21
Keppkinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 78, 435968, 436178, 436503, 436518, 530825, G01N 100, G01N 33536, G01N 33543, G01N 33567
Patent
active
053227727
ABSTRACT:
A competitive protein binding assay for rapamycin and biologically-active metabolites, derivatives and analogues thereof in blood and other biological fluids is disclosed, wherein the binding reagent is a specific rapamycin binding protein either substantially purified from the soluble cytoplasm of target cells of rapamycin action, particularly normal or transformed lymphocytes, freshly collected or in established cell lines, or synthesized by recombinant DNA techniques. Solution phase and solid state assay systems are disclosed.
REFERENCES:
Siekierka et al., Nature, 341:755 (Oct., 1989), "Acytosolic binding protein for the immunosuppressant FK506 has peptidyl-prolyl isomerase activity but is distinct from ayelophilin".
Harding et al., Nature, 341:758 (1989), Oct., "A receptor for the immunosuppressant FK506 is a cis-trans peptidyl-prolyl isomerase".
Bierer et al., PNAS, 87:9231 (1990), Dec. "Two distinct signal transmission pathways in Tlymphocytes are inhibited by complexes formed between an immunophilin and either FK506 or rapamycin".
Bronstein et al., "1,2-Dioxetanes, Novel Chemiluminescent Enzyme Substrates, Applications to Immunoassays", Journal of Bioluminescence and Chemiluminescence 4:99, 1989.
Williams, Curtis A. and Merrill W. Chase, eds., Methods in Immunology and Immunochemistry, vol. 1, New York:Academic Press, 1967, pp. 315-348.
Coligan, John E., et al., eds., Current Protocols in Immunology, New York:John Wiley & Sons, 1991, pp. 2.7.1-2.7.9.
Bedfnarczyk, B., S. J. Soldin, I. Gasinska, M. D. Costa, & L. Perrot, 1988, Improved Receptor Assay for Measuring Digoxin Activity Clin. Chem. 34:393-397.
H. Fretz et al., Rapamycin and FK506 Binding Proteins Feb. 13, 1991, J. Am. Chem. Soc.113:1409-1411.
M. Oellerich 1984 Enzyme-Immunoassay: A Review J. Clin. Chem.-Clin. Biochem 22:895-904.
Protein purification method, Harris et al., eds. IRL Press. 1989, p. 245.
Dumont, F. J. et al., J. Immunol., 144:1418 (1990).
Bierer, B. E., et al., Proc. Nat. Acad. Sci., 87:9231 (1990).
Schreiber, S. L., Science, 251:283 (1981).
Donnelly, J. G., et al., FASEB J., 5:A730 (1991).
Children's Research Institute
Keppkinger Esther L.
Parsons Nancy J.
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