Chemistry: molecular biology and microbiology – Vector – per se
Patent
1994-01-31
1998-05-05
Ulm, John
Chemistry: molecular biology and microbiology
Vector, per se
435 697, 4352523, 4351723, C12N 1509, C12N 510, C12N 1562
Patent
active
057473340
ABSTRACT:
A novel method for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARs) is disclosed. TSARs are concatenated heterofunctional proteins, polypeptides or peptides comprising at least two functional regions: a binding domain with affinity for a ligand and a second effector peptide portion that is chemically or biologically active. In one embodiment, the heterofunctional proteins, polypeptides or peptides further comprise a linker peptide portion between the binding domain and the second active peptide portion. The linker peptide can be either susceptible or not susceptible to cleavage by enzymatic or chemical means. Novel and/or improved heterofunctional binding reagents as well as methods for using the reagents for a variety of in vitro and in vivo applications are also disclosed.
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Adey Nils B.
Fowlkes Dana M.
Kay Brian K
Sparks Andrew B.
The University of North Carolina at Chapel Hill
Ulm John
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