Random array of micro-spheres for the analysis of nucleic...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S007100, C435S174000, C435S283100, C435S287200, C435S287900, C435S288700, C422S068100, C422S082050, C436S023000, C436S024000

Reexamination Certificate

active

06916620

ABSTRACT:
A method of identifying nucleic acid samples comprising: providing a mircoarray including a substrate coated with a composition including a population of nucleic acid probe modified micro-spheres immobilized in a coating containing a gelling agent or a precursor to a gelling agent, wherein a first portion of the micro-spheres is submerged in the gelatin coating and a second portion is exposed above the gelatin coating and is substantially free of gelatin, at least one sub-population of the population micro-spheres containing an optical barcode generated from at least one colorant associated with the micro-spheres and including a nucleic acid probe sequence; contacting the array with a target nucleic acid sequence; and detecting the color barcode of the sub-population of micro-spheres due to the interaction of the probe nucleic acid sequence and the fluorescently/chemiluminescently labeled nucleic acid sample target nucleic acid sequence.

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