Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1993-03-11
1994-06-28
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 39, 435291, 435298, 435807, 435284, 422 88, 422102, C12M 134, C12Q 116
Patent
active
053246365
ABSTRACT:
A simple device functioning as a radiorespirometer is a petri dish with a modified cover for quantitating an amount of CO.sub.2 evolved by living cultured cells. The device is a plastic culture dish having a culture surface circumscribed by a continuous sidewall. A removable flat plastic cover has a flat inside face and an annular collar around the face that fits against a sidewall. The collar is maintained in substantially gas-tight relationship against the sidewall by an elastic band or tightly mating surfaces. An opening through the cover is sealed by a gas-impervious material, such as a plastic film, that can be penetrated by a needle to introduce acid into the dish for terminating culture growth and lysing the cells. A collector tube is removably secured to the inside face of the cover and contains a CO.sub.2 trapping material, such as hyamine (methylbenzethonium hydroxide). The culture medium is provided with a cell substrate that contains radioactive carbon, and radioactive CO.sub.2 produced by the cells is then collected by the hyamine in the tube. The cover is afterwards removed from the dish, and the tube is detached from the cover and placed in a liquid scintillator to quantitate the amount of radioactive CO.sub.2 contained in the liquid.
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Bartos Dagmar
Trunkey Donald D.
Vlessis Angelo A.
Beisner William H.
The State of Oregon Acting by and Through the State Board of Hig
Wityshyn Michael G.
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