Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant...
Patent
1989-08-09
1992-01-07
Maples, John S.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
530387, 530388, 530402, A61K 39395
Patent
active
050789856
ABSTRACT:
Proteins are radiolabeled with radionuclides of technetium or rhenium by a process in which the disulfide bonds of the protein are first partially reduced with stannous salts or other disulfide reducing agents, all substances other than the desired reduced protein removed, by size exclusion chromatography or other purification means, and a specified, smaller amount of pertechnetate or perrhenate reducing agent, such as a stannous salt, is added to the reduced protein in a manner such that further reduction of the protein is limited. Pertechnetate or perrhenate is then added to the mixture of the reduced protein and the pertechnetate or perrhenate reducing agent; the pertechnetate or perrhenate is reduced and becomes strongly bonded to the protein via the sulfhydryl groups previously exposed by reduction of disulfide groups. The reduced protein and pertechnetate or perrhenate reducing agent can be idefinitely stored frozen or lyophilized.
REFERENCES:
patent: 4305922 (1981-12-01), Rhodes
patent: 4311688 (1982-01-01), Burchiel et al.
patent: 4323546 (1982-04-01), Crockford et al.
patent: 4331647 (1982-05-01), Goldenberg
patent: 4348376 (1982-09-01), Goldenberg
patent: 4421735 (1983-12-01), Haber et al.
patent: 4424200 (1984-01-01), Crockford et al.
patent: 4472371 (1984-09-01), Burchiel et al.
patent: 4478815 (1984-10-01), Burchiel et al.
patent: 4479930 (1984-10-01), Hnatowich
patent: 4647445 (1987-03-01), Lees
patent: 4652440 (1987-03-01), Paik et al.
patent: 4668503 (1987-05-01), Hnatowich
patent: 4670545 (1987-06-01), Fritzberg et al.
patent: 4877868 (1989-10-01), Reno
"Three Approaches to Radiolabeling antibodies with 99mTc" by W. C. Eckelman et al., Nucl. Med. Biol., vol. 16, No. 2, pp. 171-176 (1989).
"A New Method for Protein Labeling with 99mTc" by D. Blok et al., Nucl. Med. Biol., vol. 16, No. 1, pp. 11-16 (1989).
"Covalent Attachment of Chelating Groups to Macromolecules" by G. E. Krejcarek et al., Biochemical and Biophysical Research Communications, vol. 77, pp. 581-585 (1977).
"`Bifunctional` Chelating Agents for Binding Metal Ions to Proteins" by T. G. Wensel, et al., Radioimmunoimaging and Radioimmunotherapy, pp. 185-196 (1983).
"99m TC Labeling of Proteins: Initial Evaluation of a Novel Diaminedithiol Bifunctional Chelating Agent" by K. E. Baidoo, Cancer Research, vol. 50, pp. 799-802 (1990).
"Radioimmunoimaging of Experimental Thrombi in Dogs Using Technetium-99-m Labeled Monoclonal Antibody Fragments Reactive with Human Platelets" by P. Som, et al., Journal of Nuclear Medicine, vol. 27, No. 8, pp. 1315-1320 (1986).
"Technetium-99m-Albumin" by H. S. Stern, et al., Book Chapter published by Johns Hopkins Medical Institutions, Baltimore, MD, pp. 359-375 (U.S. Atomic Energy Commission) (1966).
"Use of Fe(II) or Sn(II) Alone for TEchnetium Labeling of Albumin" by M. S. Lin, et al., Journal of Nuclear Medicine, vol. 12, pp. 204-211 (1971).
"99m Tc-Human Serum Albumin" by W. C. Eckelman, et al., Journal of Nuclear Medicine, vol. 12, pp. 707-710 (1971).
"A Rapid Chemical Method of Labeling Human Plasma Proteins with 99m Tc-Pertechnetate at pH 7.4" by D. W. Wong et al., Journal of Applied Radiation and Isotopes, vol. 29, pp. 251-253.
"A Rapid Method for Labeling IgG with 99m" by L. G. Colombetti et al., Abstract from Journal of Nuclear Medicine, Proceedings of 26th Annual Meeting, p. 652 (1979).
"A Novel Approach to Tc-99m-Labeled Monoclonal Antibodies" by A. Schwarz et al., Proceedings of 34th Annual Meeting, vol. 28, No. 4, pp. 721 (1987).
"A Rapid and Efficient Method for Labeling IgG Antibodies with Tc-99m and Comparison to Tc-99m FAB` Antibody Fragments" by K. Y. Pak et al., Abstract from Proceedings of the 36th Annual Meeting, vol. 30, No. 5, p. 793 (1989).
"Imaging of Inflammatory Arthritis with Technetium-99m-Labeled IgG" by F. Breedveld et al., Journal of Nuclear Medicine, pp. 2017-2021 (1989).
"Coupling of the 99m Technetium-Nitrido Group to Monoclonal Antibody and Use of the Complexes for the Detection of Tumors in Mice" by J. Kanellos, JNCI, vol. 77, No. 2, pp. 431-439 (1986).
"Technetium-99m Labeling of Murine Monoclonal Antibody Fragments" by Rhodes et al. Journal of Nuclear Medicine, vol. 27, No. 5 (1986, pp. 685-693.
"Technetium-99m Labeling of Murine Monoclona Antibody Fragments" by Buck A. Rhodes et al., Journal, Nucl. Med., vol. 27, pp. 685-693 (1987).
"Radioimmunoimaging of Experimental Thrombi in Dogs Using Technetium 99m Labeled Monoclonal Antibody Fragments Reactive with Human Platelets" by P. Som et al., Journal, Nucl Med., vol. 27, pp. 1315-1320 (1986).
"A Novel Approach to TC-99m Labelled Monoclonal Antibodies" by A. Schwarz and A. Steinstraber Hoechst, Journal Nucl. Med., vol. 28, p. 721 (1987).
"A Rapid and Efficient Method for Labeling IgG Antibodies with TC-99m FAB` Antibody Fragments" by K. Y. Pak et al., Journal Nucl. Med., vol. 30, p. 793 (1989).
"A TC-99m Labelled Monoclonal Antibody PR1A3 for Radioimmunoscintigraphy, RIS of Colorectal Cancer" by M. Granowska et al., Journal Nucl. Med., vol. 30, p. 748 (1989).
Maples John S.
Peacock Deborah A.
RhoMed Incorporated
LandOfFree
Radiolabeling antibodies and other proteins with technetium or r does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Radiolabeling antibodies and other proteins with technetium or r, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Radiolabeling antibodies and other proteins with technetium or r will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-820521