Radioisotopic assay and binder therefor

Details Radioisotopic assay and binder therefor Radioisotopic assay and binder therefor

1974-03-18

1976-08-03

Sebastian, Leland A.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Attached to antibody or antibody fragment or immunoglobulin;...

23230B, 424 1, 424 12, G21H 502, G01T 116, A61K 4300

Patent

active

039729915

ABSTRACT:
There is provided a rapid and less costly radioisotopic assay for measuring the concentration of folate in blood serum. This procedure utilizes .sup.3 H-pteroylmonoglutamate, unlabeled 5-methyl-tetrahydrofolic acid, and a partially purified folate binder, such as for example a folate binder extracted from hog kidney. The procedure involves radioisotopically relating the bound amounts of a labeled folate and a known folate at various concentrations of the known folate in a system containing a predetermined amount of the labeled folate, a predetermined amount of the binder factor for the folates, and a predetermined amount of defolated test serum.

REFERENCES:
"Microbiologic Determination of Folic Acid Derivatives in Blood", vol. 20, No. 5, Nov., 1962, RB145.B6. pp. 609-616.

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