Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1980-11-17
1983-05-10
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 70, 435317, 536 27, C12P 2100, C12P 2102, C12N 100
Patent
active
043830331
ABSTRACT:
Radioiodination of aminoacyl-transfer RNAs with either .sup.125 I or .sup.131 I resulted in the preferential addition of radioiodine to bound tyrosine. The radioiodinated tyrosyl-tRNA was 10-fold purified by chromatography and was found to consist essentially of monoiodo- and diiodo-tRNA in approximately equal isotopic proportions. The radioiodinated tyrosine was readily incorporated into proteins synthesized in broken cell reactions.
The specific activity of the iodine-labeled proteins can be modulated up to the highest theoretical value (radioiodine in each tyrosine) yielding products that are valuable for use in immunoassay procedures in research and in the determination of proteins of clinical importance. In addition to the mature proteins (processed by natural post-synthetic reactions) the product proteins can comprise prohormone-type molecules which represent the precursor of normal circulating forms of hormones. The .sup.125 I-protein, -hormone, or -prohormone can be employed advantageously in diagnosis to provide marker compounds for chromatographic detection, immunoprecipitation, and serum clearance testing.
REFERENCES:
Scherberg et al.; J. Biol. Chem. 253, 1773 (1978).
Pelham et al., Eur. J. Biochem. 67, 247 (1976).
Clark et al., Methods Enzymol. 30 F, 754 (1974).
Brown et al., Biochim. Biophys. Acta 148, 423 (1967).
Woodward et al., Methods Enzymol. 30 F, 740 (1974).
Cartouzou et al.; Biochem. Biophys. Res. Comm. 15, 82 (1964).
Martinell James
Tanenholtz Alvin E.
Teplitz Jerome M.
University Patents Inc.
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