Radioimmunological determination of procollagen (type III) and p

Details Radioimmunological determination of procollagen (type III) and p Radioimmunological determination of procollagen (type III) and p

1979-04-13

1982-01-26

Nucker, Christine M.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 260112B, 424 12, G01N 3356, G01N 3358, G01T 100, G01N 3354

Patent

active

043128530

ABSTRACT:
For the radioimmunological determination of procollagen (type III) and procollagen peptide (type III) a certain amount of radioactively tagged procollagen (type III) or procollagen peptide (type III) and a highly specific anti-procollagen (type III) serum or anti-procollagen peptide (type III) serum are brought to reaction together with a sample of an unknown content of procollagen (type III) or procollagen peptide (type III), the formed antigen-antibody complex is separated, desirably by the addition of a second antibody against the highly specific antiserum, and the radioactivity of the complex or of the supernatant liquid is measured. Highly purified procollagen peptide (type III) suitable for this purpose is obtained by the degradation of tissue or body fluids with collagenase and purification by immune adsorption and chromatography.

REFERENCES:
Rohde et al., Chem. Abstracts, vol. 89, Jul. 3, 1978, Abstract #2657f.
Nowack et al., Chem. Abstracts, vol. 86, Jan. 31, 1977, Abstract #27023x.
Blackwell et al., Biochem. Biophys. Res. Comm., vol. 75, No. 1, 1977, pp. 94-101.

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