Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1978-06-22
1980-03-25
Padgett, Benjamin R.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
260112B, G01N 3316, A61K 4300, C07G 700
Patent
active
041950739
ABSTRACT:
A method of isolating alpha.sub.1 -fetoprotein sufficiently pure to be useful in radioimmunoassay procedures is described. The method comprises the procedures and techniques of affinity chromatography, blue dextran adsorption chromatography and polyacrylamide gel electrophoresis. Wherein a certain degree of reduction in kind and/or quantity of contaminants is not achieved by the first two procedures, gel filtration and mixed ion exchange cellulose chromatography are carried out in preparation for polyacrylamide gel electrophoresis. Monkey alpha.sub.1 -fetoprotein very similar to human cord blood alpha.sub.1 -fetoprotein by amino acid composition and physical properties and of sufficient purity to be useful in human radioimmunoassay procedures is obtained by the disclosed method.
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Gould George M.
Hoffmann-La Roche Inc.
Nucker Christine M.
Padgett Benjamin R.
Saxe Jon S.
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