Radioimmunoassay for isoenzymes of creative kinase having a B-su

Details Radioimmunoassay for isoenzymes of creative kinase having a B-su Radioimmunoassay for isoenzymes of creative kinase having a B-su

1979-08-01

1981-05-12

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

23230B, 424 1, 424 15, 424 12, 424 85, 435188, C12Q 166

Patent

active

042672716

ABSTRACT:
Methods and materials are disclosed for quantitative detection of isoenzymes of creatine kinase having a B-subunit by radioimmunoassay competitive displacement techniques with antibodies to human MB CK. The antibodies are specific for MB and BB creatine kinase and have no cross-reactivity with MM creatine kinase. Radioisotopically labelled isoenzymes with of creatine kinase having the same affinity for antibodies raised against human MB CK are also disclosed, which with the aforementioned antibodies provide a highly sensitive radioimmunoassay capable of measuring picomolar amounts of MB CK or BB CK.

REFERENCES:
Robert Roberts et al., Science, vol. 194, pp. 855-857, 1976. _
John B. Armstrong et al., Journal of Biological Chemistry, vol. 252, No. 10, pp. 3105-3111; 1977. _

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